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dc.contributor.advisorCampuzano, Sonsoles-
dc.contributor.authorNavascués, Joaquín de-
dc.descriptionTesis Doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura 20-07-2007en_US
dc.description.abstract[BACKGROUND] In Drosophila, the notum territory of the wing disc is specified during the second larval instar. At this stage, notum formation depends on EGFR signalling pathway in the proximal part of the disc. This EGFR activity allows the growth of the notum primordium and activates the expression of the members of the iroquois complex (Iro-C), which endow the cells of the notum anlage with notum identity. Here we have characterized the function in this process of the LIM-HD gene tailup (tup = islet). This gene has been categorized as a prepattern gene that antagonizes formation of sensory bristles on the notum of Drosophila. This function apparently relies on inhibitory physical interactions of the Tup LIM domains with both Chip and Pnr, which are important activators of the expression of the proneural achaete-scute genes.en_US
dc.description.abstract[RESULTS] We show that tup is expressed from the early second instar on and that its activity is required during the specification of the notum territory. Absence of tup function causes cells of this anlage to upregulate different wing-hinge genes and to lose expression of some notum genes. Consistently, these cells differentiate hinge structures or modified notum cuticle. The LIM-HD cofactors Chip and Ssdp are also necessary for notum specification; cells in the notum that lack any of these cofactors may express wing-hinge markers and lose notum markers, or even give rise to adults bearing ectopic hinge structures. This suggests that Tup acts in this process in a complex with Chip and Ssdp. We have evaluated the relative capacity of tup and the Iro-C member araucan to impose a notum developmental fate on other territories. We find that overexpression of tup, together with araucan, a pronotum gene member of the Iro-C, synergistically reinforces the weak capacity of either gene overexpressed singly to induce ectopic notum-like development. We have analysed the effect of several signalling pathways on tup expression, and identified the Dpp pathway as a positive regulator, while those of Wg, EGFR, PVR and Hh have no direct role in tup regulation.en_US
dc.description.abstract[CONCLUSION] Our data support a model in which the EGFR and Dpp signalling pathways, with their respective downstream genes Iro-C and tup, converge and cooperate to commit cells to the notum developmental fate.en_US
dc.format.extent44039560 bytes-
dc.publisherUniversidad Autónoma de Madriden_US
dc.subjectGenética del desarrolloen_US
dc.titleFunción del factor LIM-HD Tailup en el notum de "Drosophila"en_US
dc.typetesis doctoralen_US
dc.description.peerreviewedPeer revieweden_US
Appears in Collections:(CBM) Tesis
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