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Protection of human HepG2 cells against oxidative stress by cocoa phenolic extract

AuthorsMartín, M. Angeles; Ramos, Sonia ; Mateos, Raquel ; Serrano, A. B. G.; Izquierdo-Pulido, M.; Bravo, Laura ; Goya, Luis
Issue Date2008
PublisherAmerican Chemical Society
CitationJournal of Agricultural and Food Chemistry 56: 7765- 7772 (2008)
AbstractCocoa is a rich source of flavanols and procyanidin oligomers with antioxidative properties, providing protection against oxidation and nitration. The present study investigated the potential protective effect of a polyphenolic extract from cocoa on cell viability and antioxidant defenses of cultured human HepG2 cells submitted to oxidative stress induced by tert-butylhydroperoxide (t-BOOH). Pretreatment of cells with 0.05-50 μg/mL of cocoa polyphenolic extract (CPE) for 2 or 20 h completely prevented cell damage and enhanced activity of antioxidant enzymes induced by a treatment with t-BOOH. Moreover, lower levels of GSH caused by t-BOOH in HepG2 cells were partly recovered by a pretreatment with CPE. Increased reactive oxygen species (ROS) induced by t-BOOH was dose-dependently prevented when cells were pretreated for 2 or 20 h with CPE. These results show that treatment of HepG2 in culture with CPE (within the physiological range of concentrations) confers a significant protection against oxidation to the cells. © 2008 American Chemical Society.
Identifiersdoi: 10.1021/jf801744r
issn: 0021-8561
Appears in Collections:(IF) Artículos
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