English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/83239
Share/Impact:
Statistics
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Stem cells isolated from adipose tissue of obese patients show changes in their transcriptomic profile that indicate loss in stemcellness and increased commitment to an adipocyte-like phenotype

AuthorsOñate, Blanca; Vilahur, Gemma; Camino-López, Sandra; Díez-Caballero, Alberto; Ballesta-López, Carlos; Ybarra, Juan; Moscatiello, Fabrizio; Herrero, Javier; Badimón Maestro, Lina
Issue Date16-Sep-2013
PublisherBioMed Central
CitationBMC Genomics. 2013 Sep 16;14(1):625
AbstractAbstract Background The adipose tissue is an endocrine regulator and a risk factor for atherosclerosis and cardiovascular disease when by excessive accumulation induces obesity. Although the adipose tissue is also a reservoir for stem cells (ASC) their function and “stemcellness” has been questioned. Our aim was to investigate the mechanisms by which obesity affects subcutaneous white adipose tissue (WAT) stem cells. Results Transcriptomics, in silico analysis, real-time polymerase chain reaction (PCR) and western blots were performed on isolated stem cells from subcutaneous abdominal WAT of morbidly obese patients (ASCmo) and of non-obese individuals (ASCn). ASCmo and ASCn gene expression clustered separately from each other. ASCmo showed downregulation of “stemness” genes and upregulation of adipogenic and inflammatory genes with respect to ASCn. Moreover, the application of bioinformatics and Ingenuity Pathway Analysis (IPA) showed that the transcription factor Smad3 was tentatively affected in obese ASCmo. Validation of this target confirmed a significantly reduced Smad3 nuclear translocation in the isolated ASCmo. Conclusions The transcriptomic profile of the stem cells reservoir in obese subcutaneous WAT is highly modified with significant changes in genes regulating stemcellness, lineage commitment and inflammation. In addition to body mass index, cardiovascular risk factor clustering further affect the ASC transcriptomic profile inducing loss of multipotency and, hence, capacity for tissue repair. In summary, the stem cells in the subcutaneous WAT niche of obese patients are already committed to adipocyte differentiation and show an upregulated inflammatory gene expression associated to their loss of stemcellness.
URIhttp://hdl.handle.net/10261/83239
Identifiershttp://dx.doi.org/10.1186/1471-2164-14-625
Appears in Collections:(CIC) Artículos
Files in This Item:
File Description SizeFormat 
1471-2164-14-625.xml81,88 kBXMLView/Open
1471-2164-14-625-S1.TIFF55,34 kBTIFFThumbnail
View/Open
1471-2164-14-625-S2.TIFF759,14 kBTIFFThumbnail
View/Open
1471-2164-14-625-S3.TIFF300,23 kBTIFFThumbnail
View/Open
1471-2164-14-625.pdf704,89 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.