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dc.contributor.authorPalomares Rius, Juan E.-
dc.contributor.authorJones, John T.-
dc.contributor.authorCock, Peter J.-
dc.contributor.authorCastillo, Pablo-
dc.contributor.authorBlok, Vivian C.-
dc.date.accessioned2013-10-02T07:50:15Z-
dc.date.available2013-10-02T07:50:15Z-
dc.date.issued2013-04-
dc.identifier.citationParasitology 140(04): 445-454 (2013)es_ES
dc.identifier.issn0031-1820-
dc.identifier.urihttp://hdl.handle.net/10261/83101-
dc.description.abstractThe potato cyst nematodes (PCN) Globodera pallida and G. rostochiensis are major pests of potatoes. The G. pallida (and G. rostochiensis) life cycle includes both diapause and quiescent stages. Nematodes in dormancy (diapause or quiescent) are adapted for long-term survival and are more resistant to nematicides. This study analysed the mechanisms underlying diapause and quiescence. The effects of several compounds (8Br-cGMP, oxotremorine and atropine) on the activation of hatching were studied. The measurements of some morphometric parameters in diapaused and quiescent eggs after exposure to PRD revealed differences in dorsal gland length, subventral gland length and dorsal gland nucleolus. In addition, the expression of 2 effectors (IVg9 and cellulase) was not induced in diapaused eggs in water or PRD, while expression was slightly induced in quiescent eggs. Finally, we performed a comparative study to identify orthologues of C. elegans diapause related genes in plant-parasitic nematodes (G. pallida, Meloidogyne incognita, M. hapla and Bursaphelenchus xylophilus). This analysis suggested that it was not possible to identify G. pallida orthologues of the majority of C. elegans genes involved in the control of dauer formation. All these data suggest that G. pallida may use different mechanisms to C. elegans in regulating the survival stage.es_ES
dc.description.sponsorshipThe authors thank the Spanish Ministry of Education for funding to the fi rst author under the ‘ Ayudas para la movilidadpostdoctoralen centrosextranjeros ’ scheme. The James Hutton Institute receives funding from the Scottish Government. Statistical advice from Jim McNicol is gratefully acknowledged (BIOSS, UK).es_ES
dc.language.isoenges_ES
dc.publisherCambridge University Presses_ES
dc.rightsopenAccesses_ES
dc.subjectHatchinges_ES
dc.subjectcGMPes_ES
dc.subjectMuscarinic pathwayes_ES
dc.subjectPotato cyst nematodeses_ES
dc.subjectRoot diffusatees_ES
dc.titleActivation of hatching in diapaused and quiescent Globodera pallidaes_ES
dc.typeartículoes_ES
dc.identifier.doi10.1017/S0031182012001874-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp:dx.doi.org/10.1017/S0031182012001874es_ES
dc.identifier.e-issn1469-8161-
dc.embargo.terms2014-04-01es_ES
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