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Title

Thyroid hormones regulate melanogenesis in zebrafish

AuthorsGuillot, Raúl ; Rotllant, Josep ; Cerdá-Reverter, José Miguel
Issue Date2012
AbstractThyroid hormones regulate a wide spectrum oí functions in the fish physiology. Recent investigations have related thyroidal system with the regulabon of physiological color patterning in fish. Commonly, zebrafish embryos are treated with anti-thyroidal compounds, as phenylthiourea (PTU), to inhibit melanogenesis by blocking all tyrosinase-depending steps in the melanin synthesis. Similarly, tyrosinase gene expression is down-regulated alsoin ernbryos showing low intracellular triiodothyronine (T3) availability. Accordingly, exogenous T3 cause increased pigmentation in zebrafish embryos thus suggesting that thyroidal system can inhibit early larval pigment pattern in zebrafish. On the contrary, our experiments demonstrate that adult zebrafish fcd with T3-supplemenled diels exhibit asevere disrupbon of the physiological pigment pattern aHer 15 days. The stripped pigment pattern of the zebrafish was much less evident in treated anirnals. However, animals fed back with non-supplemented díets recovered fue regular stripped phenotypeafter 2 weeks. In order to characterize the molecular mechanisms, we analyze the expression oí genes involved in the differentiahon, mah¡ration and survival of the melanocytes as well as the genes involved in the melanin synthesis pathways. Treated fish displayed asevere down regulation oí the melanogenenic pathways. Therefore, 13 down-regulate the expression of sorne gene encod ing melanogenic enzymes but T3 effects are sex dependent. In addition, our results demonstrate a sexdependent expression of different melanogenesis-related genes.
URIhttp://hdl.handle.net/10261/83084
Appears in Collections:(IIM) Comunicaciones congresos
(IATS) Comunicaciones congresos
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