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The interference of galaxolide with endogenous and xenobiotic metabolizing enzymes in sea bass

AuthorsDimastrogiovanni, Giorgio; Fernandes, Denise; Blázquez, Mercedes ; Porte Visa, Cinta
Issue Date2012
PublisherAsociación Ibérica de Endocrinología Comparada
Citation8º Congreso de la Asociación Ibérica de Endocrinología Comparada (2011)
AbstractPolycyclic musks are extensively used as fragrance additives in detergents, cleaning agents, shampoos and deodorants. The wide occurrence of these substances in the aquatic environment and their high bioconcentration potential raise concerns about likely effects on aquatic species. This study was designed to investigate the in vivo interactions of the most widely used musk, galaxolide (GAL), with fish enzymatic systems involved in both xenobiotic and endogenous metabolism. Sea bass fish were intraperitoneally injected with 50 mg/kg b.w. of GAL, and the effects on different cytochrome P450 isoenzymes (CYP1A, CYP3A, CYP17 and CYP11β) and the phase II enzyme UDP-glucuronosyltransferase (UGT) were investigated 24, 48 and 96 h post-injection. In addition, a group of fish injected with 50 mg/kg bw of ketoconazole (KCZ) was used as a positive control, since KCZ inhibits several CYP isoenzymes. The results show that 24h after GAL injection, hepatic CYP1A and CYP3A activities remained unaltered, while a significant induction of the glucuronidation of pNP (UGT activity) was observed. Regarding testicular synthesis of androgens, GAL significantly reduced the formation of androstenedione by inhibiting CYP17 activity 24h after injection, as well as the synthesis of βAD by inhibiting CYP11β activity 96h after injection. In fish treated with KCZ, the positive control for CYP isoenzyme inhibition, the activity of all the studied enzymes was affected. The results show that in sea bass KCZ exhibited a higher ability than GAL of interfering with phase I and phase II enzymes. Moreover, they suggest that GAL may interfere with the synthesis of active androgens in fish by the inhibition of CYP17 and CYP11β catalytic activities.
DescriptionPoster presentado en el 8º Congreso de la Asociación Ibérica de Endocrinología Comparada celebrado en Madrid del 5 al 7 septiembre 2011
Appears in Collections:(IDAEA) Comunicaciones congresos
(IATS) Comunicaciones congresos
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