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Title

Temperature-dependent hypermutational phenotype in recA mutants of Thermus thermophilus HB27

AuthorsCastán, Pablo; Casares, Lorena; Barbé, Jordi; Berenguer, José
KeywordsThermus thermophilus
Issue Date2003
PublisherAmerican Society for Microbiology
CitationJournal of Bacteriology, 2003, p. 4901-4907, Vol. 185, No. 16
AbstractThe recA gene from Thermus thermophilus HB27 was cloned and engineered to obtain insertion (recA::kat) and deletion (delta-recA) derivatives. Transcription of recA in this extreme thermophile was induced by mitomycin C, leading to the synthesis of a monocistronic mRNA. This DNA damage-mediated induction was dependent on the integrity of recA. In addition to UV sensitivity, the recA mutants of T. thermophilus showed severe pleiotropic defects, ranging from irregular nucleoid condensation and segregation to a dramatic reduction in viability during culture. An increase in the frequency of both carotenoidless and auxotrophic mutants within surviving cells of the delta-recA strain indicated a high mutation rate. As RecA is not required for plasmid transformation, we have used the alpha-lacZ gene fragment and the ampicillin resistance gene from Escherichia coli as passenger reporters to confirm such high mutation rates. Our data support the idea that the absence of RecA results in a hypermutational phenotype in T. thermophilus. Furthermore, a direct relationship is deduced between the growth temperature and mutation rate, which finally has a deleterious effect on cell survival in the absence of RecA.
Publisher version (URL)http://dx.doi.org/10.1128/JB.185.16.4901-4907.2003
URIhttp://hdl.handle.net/10261/8289
DOI10.1128/JB.185.16.4901-4907.2003
ISSN0021-9193
E-ISSN1098-5530
Appears in Collections:(CBM) Artículos
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