English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/8284
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

Title

Efficient virus extinction by combinations of a mutagen and antiviral inhibitors

AuthorsPariente, Nonia; Sierra, Saleta; Lowenstein, Pedro R.; Domingo, Esteban
KeywordsFoot-and-mouth disease virus
Issue Date2001
PublisherAmerican Society for Microbiology
CitationJournal of Virology, 2001, p. 9723-9730, Vol. 75, No. 20
AbstractThe effect of combinations of the mutagenic base analog 5-fluorouracil (FU) and the antiviral inhibitors guanidine hydrochloride (G) and heparin (H) on the infectivity of foot-and-mouth disease virus (FMDV) in cell culture has been investigated. Related FMDV clones differing up to 106-fold in relative fitness in BHK-21 cells have been compared. Systematic extinction of intermediate fitness virus was attained with a combination of FU and G but not with the mutagen or the inhibitor alone. Systematic extinction of high-fitness FMDV required the combination of FU, G, and H. FMDV showing high relative fitness in BHK-21 cells but decreased replicative ability in CHO cells behaved as a low-fitness virus with regard to extinction mutagenesis in CHO cells. This confirms that relative fitness, rather than a specific genomic sequence, determines the FMDV response to enhanced mutagenesis. Mutant spectrum analysis of several genomic regions from a preextinction population showed a statistically significant increase in the number of mutations compared with virus passaged in parallel in the absence of FU and inhibitors. Also, in a preextinction population the types of mutations that can be attributed to the mutagenic action of FU were significantly more frequent than other mutation types. The results suggest that combinations of mutagenic agents and antiviral inhibitors can effectively drive high-fitness virus into extinction.
Publisher version (URL)http://dx.doi.org/10.1128/JVI.75.20.9723-9730.2001
URIhttp://hdl.handle.net/10261/8284
DOIhttp://dx.doi.org/10.1128/JVI.75.20.9723-9730.2001
ISSN0022-538X
E-ISSN1098-5514
Appears in Collections:(CBM) Artículos
Files in This Item:
File Description SizeFormat 
NPariente_JVirol_9723.pdf134,19 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.