English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/8284
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 106 veces en Web of Knowledge®  |  Pub MebCentral Ver citas en PubMed Central  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar EndNote (RIS)Exportar EndNote (RIS)
Título

Efficient virus extinction by combinations of a mutagen and antiviral inhibitors

Autor Pariente, Nonia; Sierra, Saleta; Lowenstein, Pedro R.; Domingo, Esteban
Palabras clave Foot-and-mouth disease virus
Fecha de publicación 2001
EditorAmerican Society for Microbiology
Citación Journal of Virology, 2001, p. 9723-9730, Vol. 75, No. 20
ResumenThe effect of combinations of the mutagenic base analog 5-fluorouracil (FU) and the antiviral inhibitors guanidine hydrochloride (G) and heparin (H) on the infectivity of foot-and-mouth disease virus (FMDV) in cell culture has been investigated. Related FMDV clones differing up to 106-fold in relative fitness in BHK-21 cells have been compared. Systematic extinction of intermediate fitness virus was attained with a combination of FU and G but not with the mutagen or the inhibitor alone. Systematic extinction of high-fitness FMDV required the combination of FU, G, and H. FMDV showing high relative fitness in BHK-21 cells but decreased replicative ability in CHO cells behaved as a low-fitness virus with regard to extinction mutagenesis in CHO cells. This confirms that relative fitness, rather than a specific genomic sequence, determines the FMDV response to enhanced mutagenesis. Mutant spectrum analysis of several genomic regions from a preextinction population showed a statistically significant increase in the number of mutations compared with virus passaged in parallel in the absence of FU and inhibitors. Also, in a preextinction population the types of mutations that can be attributed to the mutagenic action of FU were significantly more frequent than other mutation types. The results suggest that combinations of mutagenic agents and antiviral inhibitors can effectively drive high-fitness virus into extinction.
Versión del editorhttp://dx.doi.org/10.1128/JVI.75.20.9723-9730.2001
URI http://hdl.handle.net/10261/8284
DOI10.1128/JVI.75.20.9723-9730.2001
ISSN0022-538X (print)
1098-5514 (online)
Aparece en las colecciones: (CBM) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
NPariente_JVirol_9723.pdf134,19 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.