English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/8272
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 37 veces en Web of Knowledge®  |  Pub MebCentral Ver citas en PubMed Central  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar bibText (RIS)Exportar csv (RIS)
Título

C-reactive protein exerts angiogenic effects on vascular endothelial cells and modulates associated signalling pathways and gene expression

Autor Turu, Marta M.; Slevin, Mark; Matou, Sabine; West, David; Rodríguez, Cristina; Luque, Ana; Grau-Olivares, Marta; Badimón, Lina; Martínez-González, José; Krupinski, Jerzy
Palabras clave C-reactive protein
CRP
Vascular endothelial cells
Fecha de publicación 2-sep-2008
EditorBioMed Central
Citación BMC Cell Biology 2008, 9:47
ResumenBackground Formation of haemorrhagic neovessels in the intima of developing atherosclerotic plaques is thought to significantly contribute to plaque instability resulting in thrombosis. C-reactive protein (CRP) is an acute phase reactant whose expression in the vascular wall, in particular, in reactive plaque regions, and circulating levels increase in patients at high risk of cardiovascular events. Although CRP is known to induce a pro-inflammatory phenotype in endothelial cells (EC) a direct role on modulation of angiogenesis has not been established. Results Here, we show that CRP is a powerful inducer of angiogenesis in bovine aortic EC (BAEC) and human coronary artery EC (HCAEC). CRP, at concentrations corresponding to moderate/high risk (1–5 μg/ml), induced a significant increase in proliferation, migration and tube-like structure formation in vitro and stimulated blood vessel formation in the chick chorioallantoic membrane assay (CAM). CRP treated with detoxi-gel columns retained such effects. Western blotting showed that CRP increased activation of early response kinase-1/2 (ERK1/2), a key protein involved in EC mitogenesis. Furthermore, using TaqMan Low-density Arrays we identified key pro-angiogenic genes induced by CRP among them were vascular endothelial cell growth factor receptor-2 (VEGFR2/KDR), platelet-derived growth factor (PDGF-BB), notch family transcription factors (Notch1 and Notch3), cysteine-rich angiogenic inducer 61 (CYR61/CCN1) and inhibitor of DNA binding/differentiation-1 (ID1). Conclusion This data suggests a role for CRP in direct stimulation of angiogenesis and therefore may be a mediator of neovessel formation in the intima of vulnerable plaques.
Descripción Additional files: Applied bio systems codes.
Versión del editorhttp://dx.doi.org/10.1186/1471-2121-9-47
URI http://hdl.handle.net/10261/8272
DOI10.1186/1471-2121-9-47
ISSN1471-2121
Aparece en las colecciones: (CIC) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
1471-2121-9-47.pdf2,27 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.