English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/8269
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


Subcellular forms and biochemical events triggered in human cells by HCV polyprotein expression from a viral vector

AuthorsVandermeeren, Andrée M.; Elena Gómez, Carmen; Patiño, Cristina; Domingo-Gil, Elena; Guerra, Susana ; González, José Manuel; Esteban, Mariano
KeywordsSubcellular forms
Biochemical events
Issue Date15-Sep-2008
PublisherBioMed Central
CitationVirology Journal 2008, 5:102
AbstractTo identify the subcellular forms and biochemical events induced in human cells after HCV polyprotein expression, we have used a robust cell culture system based on vaccinia virus (VACV) that efficiently expresses in infected cells the structural and nonstructural proteins of HCV from genotype 1b (VT7-HCV7.9). As determined by confocal microscopy, HCV proteins expressed from VT7-HCV7.9 localize largely in a globular-like distribution pattern in the cytoplasm, with some proteins co-localizing with the endoplasmic reticulum (ER) and mitochondria. As examined by electron microscopy, HCV proteins induced formation of large electron-dense cytoplasmic structures derived from the ER and containing HCV proteins. In the course of HCV protein production, there is disruption of the Golgi apparatus, loss of spatial organization of the ER, appearance of some "virus-like" structures and swelling of mitochondria. Biochemical analysis demonstrate that HCV proteins bring about the activation of initiator and effector caspases followed by severe apoptosis and mitochondria dysfunction, hallmarks of HCV cell injury. Microarray analysis revealed that HCV polyprotein expression modulated transcription of genes associated with lipid metabolism, oxidative stress, apoptosis, and cellular proliferation. Our findings demonstrate the uniqueness of the VT7-HCV7.9 system to characterize morphological and biochemical events related to HCV pathogenesis.
Publisher version (URL)http://dx.doi.org/10.1186/1743-422X-5-102
Appears in Collections:(CNB) Artículos
Files in This Item:
File Description SizeFormat 
1743-422X-5-102.pdf1,73 MBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.