English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/8269
Compartir / Impacto:
Add this article to your Mendeley library MendeleyBASE
Citado 8 veces en Web of Knowledge®  |  Pub MebCentral Ver citas en PubMed Central  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar EndNote (RIS)Exportar EndNote (RIS)

Subcellular forms and biochemical events triggered in human cells by HCV polyprotein expression from a viral vector

Autor Vandermeeren, Andrée M.; Elena Gómez, Carmen; Patiño, Cristina; Domingo-Gil, Elena; Guerra, Susana; González, José Manuel; Esteban, Mariano
Palabras clave Subcellular forms
Biochemical events
Fecha de publicación 15-sep-2008
EditorBioMed Central
Citación Virology Journal 2008, 5:102
ResumenTo identify the subcellular forms and biochemical events induced in human cells after HCV polyprotein expression, we have used a robust cell culture system based on vaccinia virus (VACV) that efficiently expresses in infected cells the structural and nonstructural proteins of HCV from genotype 1b (VT7-HCV7.9). As determined by confocal microscopy, HCV proteins expressed from VT7-HCV7.9 localize largely in a globular-like distribution pattern in the cytoplasm, with some proteins co-localizing with the endoplasmic reticulum (ER) and mitochondria. As examined by electron microscopy, HCV proteins induced formation of large electron-dense cytoplasmic structures derived from the ER and containing HCV proteins. In the course of HCV protein production, there is disruption of the Golgi apparatus, loss of spatial organization of the ER, appearance of some "virus-like" structures and swelling of mitochondria. Biochemical analysis demonstrate that HCV proteins bring about the activation of initiator and effector caspases followed by severe apoptosis and mitochondria dysfunction, hallmarks of HCV cell injury. Microarray analysis revealed that HCV polyprotein expression modulated transcription of genes associated with lipid metabolism, oxidative stress, apoptosis, and cellular proliferation. Our findings demonstrate the uniqueness of the VT7-HCV7.9 system to characterize morphological and biochemical events related to HCV pathogenesis.
Versión del editorhttp://dx.doi.org/10.1186/1743-422X-5-102
URI http://hdl.handle.net/10261/8269
Aparece en las colecciones: (CNB) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
1743-422X-5-102.pdf1,73 MBAdobe PDFVista previa
Mostrar el registro completo

NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.