English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/82689
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

15N immobilization in forest soil: a sterilization experiment coupled with 15CPMAS NMR spectroscopy

AuthorsMorier, I.; Schleppi, P.; Siegwolf, R.; Knicker, Heike ; Guenat, C.
Issue DateJun-2008
PublisherBritish Society of Soil Science
CitationEuropean Journal of Soil Science 59 (3): 467–475 (2008)
AbstractIn temperate forests, soils are the main sink for atmospheric N deposition. The main processes proposed for N retention are microbial and abiotic immobilization in soil organic matter. The relative importance of these processes as well as the kind of resulting chemical compounds are not totally understood. We carried out a laboratory incubation of Hg-sterilized and non-sterilized organic and organo-mineral soil horizons, labelled with either 15NO3− or 15NH4+. The labelled samples were incubated for 1 hour, 1 day, or 6 days, then subjected to K2SO4 extraction and analysed with 15N CPMAS NMR spectroscopy. N immobilization was already effective in all samples and treatments after 1 hour. The corresponding NMR spectra showed that part of the immobilized 15N was already incorporated into an amide structure. In the sterilized soils labelled with 15NH4+, the tracer was rapidly and largely immobilized by an unknown process related to the presence of Hg. In the sterilized soils labelled with 15NO3−, between one-third and one-half of the added tracer was immobilized during the first hour and only 10% more over the 6 days. These results suggest that the sterilization was incomplete at first, allowing relatively great microbial immobilization during the first hour. By contrast, over a longer time, NO3− immobilization was significantly reduced to a level corresponding to an abiotic process as Hg sterilization became more effective. Even if the low signal-to-noise ratio precluded quantitative 15N NMR measurements, we showed that the amide-peptide signal, considered as a biotic signature, was dominant in all cases.
Description9 páginas, 6 figuras, 40 referencias.
Publisher version (URL)http://dx.doi.org/10.1111/j.1365-2389.2007.00998.x
URIhttp://hdl.handle.net/10261/82689
DOI10.1111/j.1365-2389.2007.00998.x
ISSN1351-0754
E-ISSN1365-2389
Appears in Collections:(IRNAS) Artículos
Files in This Item:
File Description SizeFormat 
15N_inmobilization_forest.pdf287,61 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.