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Title

Cerebral activation by fasting induces lactate accumulation in the hypothalamus

AuthorsViolante, Inês R.; Rodrigues, Tiago B.; Righi, Valeria; Nieto-Charques, Laura ; Cerdán, Sebastián
Issue Date2009
PublisherWiley-Liss
CitationMagnetic Resonance in Medicine 62(2): 279-283 (2009)
AbstractCarbon-13 (13C) high-resolution magic angle spinning (HR-MAS) spectroscopy was used to investigate the neuroglial coupling mechanisms underlying appetite regulation in the brain of C57BL/6J mice metabolizing [1-13C]glucose. Control fed or overnight fasted mice received [1-13C]glucose (20 μmol/g intraperitoneally [i.p.]), 15 min prior to brain fixation by focused microwaves. The hypothalamic region was dissected from the rest of the brain and 13C HR-MAS spectra were obtained from both biopsies. Fasting resulted in a significant increase in hypothalamic [3-13C]lactate and [2-13C]γ-aminobutyric acid (GABA) relative to the remaining brain. Administration of the orexigenic peptide ghrelin (0.3 nmol/g i.p.) did not increase hypothalamic [3-13C] lactate or [2-13C]GABA, suggesting that ghrelin signaling is not sufficient to elicit all the metabolic consequences of hypothalamic activation by fasting. Our results indicate that the hypothalamic regulation of appetite involves, in addition to the well-known neuropeptide signaling, increased neuroglial lactate shuttling and augmented GABA concentrations. © 2009 Wiley-Liss, Inc.
URIhttp://hdl.handle.net/10261/82628
DOI10.1002/mrm.22010
Identifiersdoi: 10.1002/mrm.22010
issn: 0740-3194
e-issn: 1522-2594
Appears in Collections:(IIBM) Artículos
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