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Anti-müllerian gene expression levels during sea bass sex differentiation and early maturation

AuthorsOtero, David ; Piferrer, Francesc ; Blázquez, Mercedes
Issue DateSep-2011
PublisherAsociación Ibérica de Endocrinología Comparada
Citation8º Congreso de la Asociación Ibérica de Endocrinología Comparada (2011)
AbstractAnti-müllerian hormone (amh) is a glycoprotein, member of the TGF-beta family, involved in early sex differentiation and gonadal development in higher vertebrates. Sex specific amh gene expression has been reported during gonadal development in several teleosts however, its role in this process is not clear. The present study investigates the involvement of amh in sea bass sex differentiation and gonadal development. Sea bass kept under natural conditions of photoperiod and temperature were periodically monitored for growth, since length is an indicator of sex differentiation in this species. At each sampling time, 20 fish were sacrificed and gonads and brains dissected for histological evaluation of sex and gene expression analysis using real-time PCR. amh expression was detected before histological sex differentiation in both organs although at low levels. The first significant increase in gonadal amh levels was found at 163 days post hatching (dph) (total length 5.5-7.5 cm), before histological sex differentiation. Moreover, a marked up-regulation of amh was found in males (100-fold higher) when compared to females after sex differentiation was completed (234 dph), pointing at its relevance in testicular differentiation and development. amh in males increased as gonadal development progressed reaching the highest values at 417 dph, coinciding with the end of the first reproductive season. These levels were higher in immature than in maturing testis, in agreement with the postulated role of amh as a repressor of gonadal maturation. In addition, high brain amh expression was found by the end of the first reproductive season although levels were still higher in gonads than in brain (100-fold higher). Other genes involved in sea bass sex differentiation were also monitored, among them gonadal aromatase, a molecular marker of ovarian differentiation. In all cases, amh showed reciprocal expression levels to those of aromatase, suggesting that amh expression can be used as a reliable molecular marker for sea bass testicular differentiation.
DescriptionPoster presentado en el 8º Congreso de la Asociación Ibérica de Endocrinología Comparada celebrado en Madrid del 5 al 7 septiembre 2011
Appears in Collections:(IATS) Comunicaciones congresos
(ICM) Comunicaciones congresos
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