English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/82458
logo share SHARE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Analysis of Class III Peroxidase Genes Expressed in Roots of Resistant and Susceptible Wheat Lines Infected by Heterodera avenae

AuthorsSimonetti, Ester; Veronico, Pasqua; Melillo, M. Teresa; Delibes, Ángeles; Andrés, Mª Fé ; López-Braña, I.
KeywordsPeroxidase Genes
Wheat Lines
Heterodera avenae
Issue Date2009
PublisherAmerican Phytopathological Society
CitationMolecular Plant-Microbe Interactions 22 (9): 1081-1092 (2009)
AbstractThe response of resistant wheat–Aegilops ventricosa introgression line H-93-8 and its susceptible parent, Triticum aestivum H-10-15, to Ha71 Spanish population of Heterodera avenae was studied to determine the changes in peroxidase gene expression during incompatible and compatible wheat–nematode interactions. Twenty peroxidase genes were characterized from both 211 expressed sequence tags and 259 genomic DNA clones. Alignment of deduced amino acid sequences and phylogenetic clustering with peroxidases from other plant species showed that these enzymes fall into seven different groups (designated TaPrx108 to TaPrx114) which represent peroxidases secreted to the apoplast by a putative N-terminal peptide signal. TaPrx111, TaPrx112, and TaPrx113 were induced by nematode infection in both genotypes but with differing magnitude and timing. TaPrx112 and TaPrx113 groups increased more in resistant than in susceptible infected lines. In addition, in situ hybridization analyses of genes belonging to TaPrx111, TaPrx112, and TaPrx113 groups revealed a more intense signal in cells close to the vascular cylinder and parenchyma vascular cells of resistant than susceptible wheat when challenged by nematodes. These data seem to suggest that wheat apoplastic peroxidases, because of their different expression in quantity and timing, play different roles in the plant response to nematode infection.
Description12 páginas, 6 figuras y 2 tablas estadísticas
Publisher version (URL)http://dx.doi.org/ 10.1094/MPMI -22-9-1081
DOI10.1094/MPMI -22-9-1081
Appears in Collections:(ICA) Artículos
Files in This Item:
File Description SizeFormat 
restringido.pdf21,67 kBAdobe PDFThumbnail
Show full item record
Review this work

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.