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Título

Thermal stability of peroxidase from Chamaerops excelsa palm tree at pH 3

Autor Zamorano, Laura S.; Barrera Vilarmau, Susana; Arellano, Juan B. ; Zhadan, Galina G.; Hidalgo Cuadrado, Nazaret; Bursakov, Sergey A.; Roig, Manuel G.; Shnyrov, Valery L.
Palabras clave Palm peroxidase
Protein stability
Differential scanning calorimetry
Circular dichroism
Fluorescence
Fecha de publicación may-2009
EditorElsevier
Citación International Journal of Biological Macromolecules 44(4): 326-332 (2009)
ResumenThe structural stability of a peroxidase, a dimeric protein from palm tree Chamaerops excelsa leaves (CEP), has been characterized by high-sensitivity differential scanning calorimetry, circular dichroism and steady-state tryptophan fluorescence at pH 3. The thermally induced denaturation of CEP at this pH value is irreversible and strongly dependent upon the scan rate, suggesting that this process is under kinetic control. Moreover, thermally induced transitions at this pH value are dependent on the protein concentration, leading to the conclusion that in solution CEP behaves as dimer, which undergoes thermal denaturation coupled with dissociation. Analysis of the kinetic parameters of CEP denaturation at pH 3 was accomplished on the basis of the simple kinetic scheme N -(k)-> D [View source], where k is a first-order kinetic constant that changes with temperature, as given by the Arrhenius equation; N is the native state, and D is the denatured state, and thermodynamic information was obtained by extrapolation of the kinetic transition parameters to an infinite heating rate.
Descripción 7 pages, 5 figures, 4 tables.-- PMID: 19428462 [PubMed].-- Printed version published May 1, 2009.
Versión del editorhttp://dx.doi.org/10.1016/j.ijbiomac.2009.01.004
URI 10261/8241
DOI10.1016/j.ijbiomac.2009.01.004
ISSN0141-8130
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