Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/82076
Share/Export:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Title

Brillouin microscopy of collagen crosslinking: Noncontact depth-dependent analysis of corneal elastic modulus

AuthorsScarcelli, G.; Kling, S.; Quijano, E.; Pineda, R.; Marcos, Susana CSIC ORCID ; Yun, S. H.
Issue Date2013
PublisherAssociation for Research in Vision and Ophthalmology
CitationInvestigative Ophthalmology and Visual Science 54: 1418- 1425 (2013)
AbstractPurpose. Corneal collagen crosslinking (CXL) is designed to halt the progression of keratoconus and corneal ectasia by inducing corneal stiffening. However, it currently is difficult to monitor and evaluate CXL outcome objectively due to the lack of suitable methods to characterize corneal mechanical properties. We validated noncontact Brillouin microscopy to quantify corneal mechanical properties before and after CXL. Methods. CXL was performed on fresh porcine eyes using various presoaking times and light doses, with or without epithelial debridement. From Brillouin maps of corneal elastic modulus, stiffness and average modulus of anterior, middle, and posterior stroma were analyzed. Corneal stiffening index (CSI) was introduced as a metric to compare the mechanical efficacy of a given CXL protocol with respect to the standard protocol (30-minute riboflavin presoak, 3 mW/cm2 ultraviolet illumination for 30 minutes). Results. Brillouin corneal stiffness increased significantly (P < 0.001) by epi-off and epi-on CXL. The increase of Brillouin modulus was depth-dependent, indicating that anterior stromal stiffening contributes the most to mechanical outcome. The increase of anterior Brillouin modulus was linearly proportional to the light dose (R2 > 0.98). Compared to the standard epi-off procedure, a typical epi-on procedure resulted in a third of stiffness increase in porcine corneas (CSI = 33). Conclusions. Brillouin microscopy allowed imaging and quantifying CXL-induced mechanical changes without contact in a depth-dependent manner at high spatial resolution. This technique may be useful to evaluate the mechanical outcomes of CXL procedures, to compare different crosslinking agents, and for real-time monitoring of CXL in clinical and experimental settings. © 2013 The Association for Research in Vision and Ophthalmology, Inc.
URIhttp://hdl.handle.net/10261/82076
DOI10.1167/iovs.12-11387
Identifiersdoi: 10.1167/iovs.12-11387
issn: 0146-0404
Appears in Collections:(CFMAC-IO) Artículos

Files in This Item:
File Description SizeFormat
Scarcelli.pdf551,94 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work

PubMed Central
Citations

84
checked on May 6, 2022

SCOPUSTM   
Citations

176
checked on May 11, 2022

WEB OF SCIENCETM
Citations

171
checked on May 13, 2022

Page view(s)

334
checked on May 18, 2022

Download(s)

198
checked on May 18, 2022

Google ScholarTM

Check

Altmetric

Dimensions


Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.