Method for the detection of minority genomes in virus quasispecies using DNA microchips

Abstract

A method for designing an individual antiviral therapy for a subject against a viral quasispecies responsible for a pathological state in said subject, comprising:

a) extracting from said subject a sample suspected to contain said viral quasispecies;

b) detecting minority genomes in a nucleic acid population of said viral quasispecies wherein said minority genomes are present in a proportion lesser than 50% of said viral quasispecies and containing at least one mutation in comparison to the majority genome of said viral quasispecies;

c) detecting the existence of nucleotidic mutations associated to the resistance to antiviral drugs in said minority genomes, and

d) designing an antiviral therapy comprising the use of one or more antiviral drugs for which neither the majority genomes nor the minority genomes present mutations associated with the virus resistance against the same.