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Use of an antisense RNA strategy to investigate the functional significance of Mn-catalase in the extreme thermophile Thermus thermophilus

AuthorsMoreno, Renata; Hidalgo, Aurelio ; Cava, Felipe ; Fernández-Lafuente, Roberto ; Manuel Guisán, José; Berenguer, José
KeywordsThermus thermophilus
Issue Date2004
PublisherAmerican Society for Microbiology
CitationJournal of Bacteriology, 2004, p. 7804-7806, Vol. 186, No. 22
AbstractThe expression of an antisense RNA revealed that an Mn-catalase was required in Thermus thermophilus for aerobic but not for anaerobic growth. The antisense system is based on the constitutive expression of a "bicistronic" transcript consisting of the kanamycin resistance gene mRNA followed by the antisense RNA against the selected target
Publisher version (URL)http://dx.doi.org/10.1128/JB.186.22.7804-7806.2004
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