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Title

Snail1 transcriptional repressor binds to its own promoter and controls its expression

AuthorsLarriba, María Jesús ; Muñoz Terol, Alberto ; Baulida, Josep; García de Herreros, Antonio
Issue Date2006
PublisherOxford University Press
CitationNucleic Acids Research 34(7): 2077-2084 (2006)
AbstractThe product of Snail1 gene is a transcriptional repressor of E-cadherin expression and an inductor of the epithelial-mesenchymal transition in several epithelial tumour cell lines. Transcription of Snail1 is induced when epithelial cells are forced to acquire a mesenchymal phenotype. In this work we demonstrate that Snail1 protein limits its own expression: Snail1 binds to an E-box present in its promoter (at - 146 with respect to the transcription start) and represses its activity. Therefore, mutation of the E-box increases Snail1 transcription in epithelial and mesenchymal cells. Evidence of binding of ectopic or endogenous Snail1 to its own promoter was obtained by chromatin immunoprecipitation (ChIP) experiments. Studies performed expressing different forms of Snail1 under the control of its own promoter demonstrate that disruption of the regulatory loop increases the cellular levels of Snail protein. These results indicate that expression of Snail1 gene can be regulated by its product and evidence the existence of a fine-tuning feed-back mechanism of regulation of Snail1 transcription. © 2006 Oxford University Press.
DescriptionFunding to pay the Open Access publication charges for this article was provided by FIS 03/0925 (to J.B.).-- et al.
Publisher version (URL)http://dx.doi.org/10.1093/nar/gkl141
URIhttp://hdl.handle.net/10261/81507
DOI10.1093/nar/gkl141
Identifiersdoi: 10.1093/nar/gkl141
issn: 0305-1048
e-issn: 1362-4962
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