Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/81405
COMPARTIR / EXPORTAR:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

Invitar a revisión por pares abierta
Título

Role of c-Src in human MCF7 breast cancer cell tumorigenesis

AutorGonzález, Lorena; Agulló-Ortuño, María Teresa CSIC; García-Martínez, José Manuel CSIC; Calcabrini, Annarica CSIC ORCID; Gamallo, Carlos; Palacios, José; Aranda, Ana CSIC ORCID ; Martín-Pérez, Jorge CSIC ORCID
Fecha de publicación2006
EditorAmerican Society for Biochemistry and Molecular Biology
CitaciónJournal of Biological Chemistry 281(30): 20851-20864 (2006)
ResumenTo study the role of c-Src in breast cancer tumorigenesis, we generated a cell line derived from MCF7 carrying an inducible dominant negative c-Src (c-SrcDN: K295M/Y527F) under tetracycline control (Tet-On system). c-SrcDN expression caused phenotypic changes, relocation of c-Src, Fak, and paxillin, and loss of correct actin fiber assembly. These alterations were coupled to increased Fak-Tyr397 autophosphorylation and to inhibition of Fak-Tyr925, p130CAS, and paxillin phosphorylation. An increased association of total Src with Fak and a decreased interaction of p130CAS and p85-PI3K with Fak were also observed. SrcDN inhibited cell attachment, spreading, and migration. Serum and EGF-induced stimulation of cell proliferation and Akt phosphorylation were also significantly reduced by SrcDN, whereas p27Kip1 expression was increased. Consistently, silencing c-Src expression by siRNA in MCF7 cells significantly reduced cell migration, attachment, spreading and proliferation. Inoculation of MCF7 cells carrying inducible SrcDN to nude mice generated tumors. However, doxycycline administration to mice significantly reduced tumorigenesis, and when doxycycline treatment was installed after tumor development, a significant tumor regression was observed. In both situations, inhibition of tumorigenesis was associated with decreased Ki67 staining and increased apoptosis in tumors. These data undoubtedly demonstrate the relevance of the Src/Fak complex in breast cancer tumorigenesis. © 2006 by The American Society for Biochemistry and Molecular Biology, Inc.
URIhttp://hdl.handle.net/10261/81405
DOI10.1074/jbc.M601570200
Identificadoresdoi: 10.1074/jbc.M601570200
issn: 0021-9258
e-issn: 1083-351X
Aparece en las colecciones: (IIBM) Artículos




Ficheros en este ítem:
Fichero Descripción Tamaño Formato
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo

CORE Recommender

SCOPUSTM   
Citations

82
checked on 13-mar-2024

WEB OF SCIENCETM
Citations

75
checked on 24-feb-2024

Page view(s)

340
checked on 17-mar-2024

Download(s)

94
checked on 17-mar-2024

Google ScholarTM

Check

Altmetric

Altmetric


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.