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Título

Method for obtaining a recombinant proteins and its utilization in the assay for for the african swine pest virus (ASPV)

AutorLópez Otín, Carlos; Pérez Freije, José María; Viñuela, Eladio
Palabras claveAfrican swine pest virus
Fecha de publicación27-jun-1991
CitaciónWO 1991/009313 A1
ResumenThe object of the method is the detection of the african swine pest virus by an immuno enzymatic assay wherein the major protein of the virus (p72) is used as antigen, said protein being obtained by expression of the gene which encodes it. The gene was identified by hybridization with oligonucleotide probes derived from structural information of the purified protein. Once its sequence of nucleotides was determined, the gene was inserted in the expression vector pAR3038, to obtain the recombinant plasmid pS72a. Said plasmid was used to transform the strain of E.coli BL21(DE3) which, after induction with IPTG, allowed the high yield production of the recombinant protein p72. The protein was purified by high resolution liquid chromatography and was directly used in antigen-antibody assays with animal serum samples in order to diagnose the disease.
DescripciónFiling Date: 1990-11-14 -- Priority Data: ES P 8904300 (1989-12-20)
URIhttp://hdl.handle.net/10261/8133
Aparece en las colecciones: (CBM) Patentes
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