Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/81007
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Interaction of Bifidobacterium bifidum LMG13195 with HT29 Cells Influences regulatory-T-cell-associated chemokine receptor expression |
Autor: | López, Patricia CSIC ORCID; González Rodríguez, Irene CSIC; Sánchez García, Borja CSIC ORCID; Ruas-Madiedo, Patricia CSIC ORCID ; Suárez, Ana; Margolles Barros, Abelardo CSIC ORCID; Gueimonde Fernández, Miguel CSIC ORCID | Fecha de publicación: | 2012 | Editor: | American Society for Microbiology | Citación: | Applied and Environmental Microbiology 78(8): 2850-2857 (2012) | Resumen: | Probiotics play an important role in the maintenance of the gastrointestinal barrier. In addition to direct effects on mucosal integrity, the interaction with the intestinal mucosa may have an active immunoregulatory effect. In the present work, we exposed HT29 intestinal epithelial cells to two Bifidobacterium species to determine their effect on gene expression profile, enterocyte monolayer integrity, and T-cell response. Bifidobacterium breve IPLA 20004 triggered a more pronounced increase in the transepithelial resistance of the enterocyte monolayer than Bifidobacterium bifidum LMG13195. The transcriptome profile of HT29 cells cultured in the presence of B. bifidum LMG13195 showed an increased expression of immune mediators and, interestingly, chemotactic molecules (CXCL10, CCL20, CXCL11 and CCL22) able to recruit lymphocytes. Since regulatory T cells (Treg cells) may express receptors for specific chemokines, we cultured peripheral blood mononuclear cells with supernatants of HT29 cells previously treated with Bifidobacterium strains and analyzed FOXP3 and CD25 Treg markers and CCR6, CXCR3, CCR4, and CCR3 expression on CD4 + lymphocytes. The proportion of CD 25high FOXP3 + cells was significantly increased after culture with B. bifidum LMG13195-conditioned HT29 supernatant. Moreover, this treatment led to the largest amount of CCR6 + CXCR3 - CCR4 + CCR3 + CD4 + cells expressing high levels of CD25, corresponding to the Treg population. These results suggest that soluble factors secreted after B. bifidum LMG13195 contact with intestinal epithelial cells favored the generation of CD4+ CD25high lymphocytes expressing chemokine receptor Treg markers, thus making possible their recruitment to the intestinal mucosa. © 2012, American Society for Microbiology. | URI: | http://hdl.handle.net/10261/81007 | DOI: | 10.1128/AEM.07581-11 | Identificadores: | doi: 10.1128/AEM.07581-11 issn: 0099-2240 e-issn: 1098-5336 |
Aparece en las colecciones: | (IPLA) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
28
checked on 28-feb-2024
SCOPUSTM
Citations
54
checked on 11-abr-2024
WEB OF SCIENCETM
Citations
47
checked on 25-feb-2024
Page view(s)
293
checked on 18-abr-2024
Download(s)
81
checked on 18-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.