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dc.contributor.authorSilvar Casao, Cristina-
dc.contributor.authorPerovic, Dragan-
dc.contributor.authorNussbaumer, Thomas-
dc.contributor.authorSpannagl, Manuel-
dc.contributor.authorUsadel, Björn-
dc.contributor.authorCasas Cendoya, Ana María-
dc.contributor.authorIgartua Arregui, Ernesto-
dc.contributor.authorOrdon, Frank-
dc.identifierdoi: 10.1371/journal.pone.0067336-
dc.identifierissn: 1932-6203-
dc.identifier.citationSilvar C, Perovic D, Nussbaumer T, Spannagl M, Usadel B, Casas A, Igartua E, Ordon F. Towards positional isolation of three quantitative trait Loci conferring resistance to powdery mildew in two Spanish barley landraces. PLoS ONE 8 (6): e67336 (2013)-
dc.description12 Pag., 3 Fig., 3 Tabl. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.-
dc.description.abstractThree quantitative trait loci (QTL) conferring broad spectrum resistance to powdery mildew, caused by the fungus Blumeria graminis f. sp. hordei, were previously identified on chromosomes 7HS, 7HL and 6HL in the Spanish barley landrace-derived lines SBCC097 and SBCC145. In the present work, a genome-wide putative linear gene index of barley (Genome Zipper) and the first draft of the physical, genetic and functional sequence of the barley genome were used to go one step further in the shortening and explicit demarcation on the barley genome of these regions conferring resistance to powdery mildew as well as in the identification of candidate genes. First, a comparative analysis of the target regions to the barley Genome Zippers of chromosomes 7H and 6H allowed the development of 25 new gene-based molecular markers, which slightly better delimit the QTL intervals. These new markers provided the framework for anchoring of genetic and physical maps, figuring out the outline of the barley genome at the target regions in SBCC097 and SBCC145. The outermost flanking markers of QTLs on 7HS, 7HL and 6HL defined a physical area of 4 Mb, 3.7 Mb and 3.2 Mb, respectively. In total, 21, 10 and 16 genes on 7HS, 7HL and 6HL, respectively, could be interpreted as potential candidates to explain the resistance to powdery mildew, as they encode proteins of related functions with respect to the known pathogen defense-related processes. The majority of these were annotated as belonging to the NBS-LRR class or protein kinase family. © 2013 Silvar et al.-
dc.description.sponsorshipProject ExpResBar, funded by the German Federal Ministry of Education and Research under grant number 0315702B within the KBBE-II call, and by the Spanish Ministry of Science and Innovation, grant number EUI2009-04075. The University of Coruña and the Deutscher Akademischer Austausch Dienst funded mobility fellowships for CS.-
dc.publisherPublic Library of Science-
dc.relation.isversionofPublisher’s version-
dc.subjectBasic leucine zipper motif-
dc.subjectChromosome 6h-
dc.subjectChromosome 7h-
dc.subjectControlled study-
dc.subjectGene identification-
dc.subjectGenetic association-
dc.subjectGenetic marker-
dc.subjectGenome analysis-
dc.subjectPlant defense-
dc.subjectPlant genome-
dc.subjectPlant pathogen interaction-
dc.subjectPowdery mildew-
dc.subjectProtein function-
dc.subjectQuantitative trait locus-
dc.subjectSequence homology-
dc.titleTowards positional isolation of three quantitative trait Loci conferring resistance to powdery mildew in two Spanish barley landraces-
dc.description.versionPeer Reviewed-
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