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Title

Enhanced direct electron transfer between laccase and hierarchical carbon microfibers/carbon nanotubes composite electrodes. Comparison of three enzyme immobilization methods

AuthorsGutiérrez Sánchez, Cristina ; Jia, Wenzhi; Beyl, Yvonne; Pita, Marcos ; Schuhmann, Wolfgang; Stoica, Leonard; Lacey, Antonio L. de
KeywordsLaccase;
Carbon nanotubes
Biofuel cell
Biocathode
Oxygen reduction reaction
Hierarchical carbon composite
Direct electron transfer
Issue Date1-Nov-2012
PublisherElsevier
CitationElectrochimica Acta 82: 218- 223 (2012)
AbstractThree immobilization protocols were investigated with respect to direct electron transfer between hierarchical carbon microfibers/carbon nanotubes composite material on graphite rod electrodes and Trametes hirsuta laccase. Immobilization was done by covalent binding of laccase to aminophenyl-modified electrodes via amide-bond formation with carboxylic acid residues or imino-bond formation with aldehyde groups introduced by oxidation of sugar residues of the enzyme's glycosylation shell. Moreover, immobilization was achieved by adsorbing laccase to electrodes hydrophilized with pyrene-hexanoic acid. High current densities for biocatalytic oxygen reduction were obtained for all immobilization strategies. The formation of the imino bonds let to the binding of laccase in close to 100% direct electron transfer configuration and consequently to the highest oxygen reduction currents.
Description18 páginas, 4 figuras
Publisher version (URL)http://dx.doi.org/10.1016/j.electacta.2011.12.134
URIhttp://hdl.handle.net/10261/80805
DOI10.1016/j.electacta.2011.12.134
ISSN0013-4686
Appears in Collections:(ICP) Artículos
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