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dc.contributor.authorSalvachúa, Davinia-
dc.contributor.authorMartínez, Ángel T.-
dc.contributor.authorTien, Ming-
dc.contributor.authorLópez-Lucendo, María F.-
dc.contributor.authorGarcía, Francisco-
dc.contributor.authorRíos, Vivian de los-
dc.contributor.authorMartínez, María Jesús-
dc.contributor.authorPrieto, Alicia-
dc.date.accessioned2013-08-14T07:08:26Z-
dc.date.available2013-08-14T07:08:26Z-
dc.date.issued2013-08-10-
dc.identifier.citationBiotechnology for Biofuels 6(1): 115(2013)-
dc.identifier.issn1754-6834-
dc.identifier.urihttp://hdl.handle.net/10261/80652-
dc.description.abstractAbstract Background Identifying new high-performance enzymes or enzyme complexes to enhance biomass degradation is the key for the development of cost-effective processes for ethanol production. Irpex lacteus is an efficient microorganism for wheat straw pretreatment, yielding easily hydrolysable products with high sugar content. Thus, this fungus was selected to investigate the enzymatic system involved in lignocellulose decay, and its secretome was compared to those from Phanerochaete chrysosporium and Pleurotus ostreatus which produced different degradation patterns when growing on wheat straw. Extracellular enzymes were analyzed through 2D-PAGE, nanoLC/MS-MS, and homology searches against public databases. Results In wheat straw, I. lacteus secreted proteases, dye-decolorizing and manganese-oxidizing peroxidases, and H2O2 producing-enzymes but also a battery of cellulases and xylanases, excluding those implicated in cellulose and hemicellulose degradation to their monosaccharides, making these sugars poorly available for fungal consumption. In contrast, a significant increase of β-glucosidase production was observed when I. lacteus grew in liquid cultures. P. chrysosporium secreted more enzymes implicated in the total hydrolysis of the polysaccharides and P. ostreatus produced, in proportion, more oxidoreductases. Conclusion The protein pattern secreted during I. lacteus growth in wheat straw plus the differences observed among the different secretomes, justify the fitness of I. lacteus for biopretreatment processes in 2G-ethanol production. Furthermore, all these data give insight into the biological degradation of lignocellulose and suggest new enzyme mixtures interesting for its efficient hydrolysis.-
dc.description.sponsorshipThis work has been carried out with funding from the Spanish project PRI-PIBAR-2011-1402 and the PEROXICATS European project.We acknowledge support by the CSIC Open Access Publication Initiative through its Unit of Information Resources for Research (URICI).-
dc.language.isoeng-
dc.publisherBioMed Central-
dc.relation.isversionofPublisher’s version-
dc.rightsopenAccess-
dc.subjectEnzymatic hydrolysis-
dc.subjectBioethanol-
dc.subjectDyP-
dc.subjectPleurotus ostreatus-
dc.subjectPhanerochaete chrysosporium-
dc.subjectLignocellulose-
dc.subjectExtracellular enzymes-
dc.titleDifferential proteomic analysis of the secretome of Irpex lacteus and other white-rot fungi during wheat straw pretreatment-
dc.typeartículo-
dc.identifier.doi10.1186/1754-6834-6-115-
dc.relation.publisherversionhttp://dx.doi.org/10.1186/1754-6834-6-115-
dc.identifier.e-issn1754-6834-
dc.date.updated2013-08-14T07:08:26Z-
dc.description.versionPeer Reviewed-
dc.rights.holderDavinia Salvachúa et al.; licensee BioMed Central Ltd.-
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