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Global DNA methylation patterns in the European sea bass exposed to different temperature profiles during embryogenesis and early larval development

AuthorsAnastasiadi, Dafni ; Díaz, Noelia ; Piferrer, Francesc
Issue DateJul-2012
PublisherSocietat Catalana de Biologia
CitationI CIBICAT: 323 (2012)
AbstractFish represent good animal models for the purposes of identifying persistent epigenetic marks which arise from different early developmental environments, and which correlate with relevant biological processes later in life. In adult European sea bass (Dicentrarchus labrax), the methylation levels of the promoter of the gonadal aromatase (cyp19a), which converts androgens into estrogens, has been shown to increase in females after exposure of larvae to high temperature. This epigenetic mechanism is involved in the masculinization of sea bass under farming conditions due to the use of elevated temperatures. In the present study, European sea bass were exposed to different combinations of temperatures during different periods covering embryogenesis or the early larval stages and were sampled at 15 days post fertilization. Global DNA methylation patterns are studied in these fish by applying a modification of the AFLP technique called Methylation Sensitive Amplified Polymorphism (MSAP). This technique allows the comparison and the detection of differences in the DNA methylation pattern profiles of groups of animals or treatments. The results contribute to better characterize the epigenetic mechanism linking temperature changes during the early developmental environment and DNA methylation of the European sea bass
DescriptionI Congrés Internacional de Biologia de Catalunya (CIBICAT): Global Questions on Advanced Biology. An international conference on interdisciplinary frontiers in biology. As part of the first Centenary of the Societat Catalana de Biologia. 9-12 July 2012, Barcelona
Publisher version (URL)http://scb-congres.espais.iec.cat/en/
Appears in Collections:(ICM) Comunicaciones congresos
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