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Genome annotation and intraviral interactome for the streptococcus pneumoniae virulent phage Dp-1

AuthorsSabri, M.; Häuser, R.; Ouellette, M.; Liu, J.; Dehbi, M.; Moeck, G.; García, Ernesto ; Titz, B.; Uetz, P.; Moineau, S.
Issue Date2011
PublisherAmerican Society for Microbiology
CitationJournal of Bacteriology 193(2) : 551- 562 (2011)
AbstractStreptococcus pneumoniae causes several diseases, including pneumonia, septicemia, and meningitis. Phage Dp-1 is one of the very few isolated virulent S. pneumoniae bacteriophages, but only a partial characterization is currently available. Here, we confirmed that Dp-1 belongs to the family Siphoviridae. Then, we determined its complete genomic sequence of 56,506 bp. It encodes 72 open reading frames, of which 44 have been assigned a function. We have identified putative promoters, Rho-independent terminators, and several genomic clusters. We provide evidence that Dp-1 may be using a novel DNA replication system as well as redirecting host protein synthesis through queuosine-containing tRNAs. Liquid chromatography-mass spectrometry analysis of purified phage Dp-1 particles identified at least eight structural proteins. Finally, using comprehensive yeast two-hybrid screens, we identified 156 phage protein interactions, and this intraviral interactome was used to propose a structural model of Dp-1.
Description12 páginas, 4 figuras, 1 tabla -- PAGS nros. 551-562
Identifiersdoi: 10.1128/JB.01117-10
issn: 0021-9193
e-issn: 1098-5530
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