English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/80544
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Lagging-strand DNA replication origins are required for conjugal transfer of the promiscuous plasmid pMV158

AuthorsLorenzo-Díaz, Fabián ; Espinosa, Manuel
Issue Date2009
PublisherAmerican Society for Microbiology
CitationJournal of Bacteriology 191(3) : 720- 727 (2009)
AbstractThe promiscuous streptococcal plasmid pMV158 is mobilizable by auxiliary plasmids and replicates by the rolling-circle mechanism in a variety of bacterial hosts. The plasmid has two lagging-strand origins, ssoA and ssoU, involved in the conversion of single-stranded DNA intermediates into double-stranded plasmid DNA during vegetative replication. Transfer of the plasmid also would involve conversion of single-stranded DNA molecules into double-stranded plasmid forms in the recipient cells by conjugative replication. To test whether lagging-strand origins played a role in horizontal transfer, pMV158 derivatives defective in one or in both sso's were constructed and tested for their ability to colonize new hosts by means of intra- and interspecies mobilization. Whereas either sso supported transfer between strains of Streptococcus pneumoniae, only plasmids that had an intact ssoU could be efficiently mobilized from S. pneumoniae to Enterococcus faecalis. Thus, it appears that ssoU is a critical factor for pMV158 promiscuity and that the presence of a functional sso plays an essential role in plasmid transfer.
Publisher version (URL)http://dx.doi.org/10.1128/JB.01257-08
URIhttp://hdl.handle.net/10261/80544
DOI10.1128/JB.01257-08
ISSN0021-9193
E-ISSN1098-5530
Appears in Collections:(CAB) Artículos
(CIB) Artículos
Files in This Item:
File Description SizeFormat 
JBact_2009_revised.pdf2,3 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.