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dc.contributor.authorGhazi, Iraj-
dc.contributor.authorFernández Arrojo, Lucía-
dc.contributor.authorGarcía-Arellano, Humberto-
dc.contributor.authorFerrer, Manuel-
dc.contributor.authorBallesteros Olmo, Antonio-
dc.contributor.authorPlou Gasca, Francisco José-
dc.date.accessioned2008-10-27T14:52:11Z-
dc.date.available2008-10-27T14:52:11Z-
dc.date.issued2007-
dc.identifier.citationJournal of Biotechnology Volume 128, Issue 1, 30 January 2007, Pages 204–211en_US
dc.identifier.urihttp://hdl.handle.net/10261/8021-
dc.description.abstractA fructosyltransferase present in Pectinex Ultra SP-L, a commercial enzyme preparation from Aspergillus aculeatus, was purified to 107-fold and further characterised. The enzyme was a dimeric glycoprotein (20% w/w carbohydrate content) with a molecular mass of around 135 kDa for the dimer. Optimal activity/stability was found in the pH range 5.0-7.0 and at 60ºC. It was stable or slightly activated (up to 1.4-fold) in the presence of reducing agents such as dithiotreitol and 2-mercaptoethanol, and detergents such as sodium dodecylsulphate and Tween 80. The enzyme was able to transfer fructosyl groups from sucrose as donor producing the corresponding series of fructooligosaccharides: 1-kestose, nystose and fructosylnystose. Using sucrose as substrate, the kcat and Km values for transfructosylating activity were 1.62  0.09 104 s-1 and 0.53  0.05 M, whereas for hydrolytic activity the corresponding values were 775  25 s-1 and 27  3 mM. At elevated sucrose concentrations, the fructosyltransferase from A. aculeatus showed a high transferase/hydrolase ratio that confers it a great potential for the industrial production of prebiotic fructooligosaccharides.en_US
dc.description.sponsorshipWe are grateful to Ramiro Martínez (Novozymes A/S, Spain) for providing us with Pectinex Ultra SP-L and for technical suggestions. We thank the AECI (Spain) and CSIC for research fellowships. Project BIO2004-03773-C00-01 from Spanish Ministry of Science and Technology supported this research.en_US
dc.format.extent329517 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.rightsopenAccessen_US
dc.subjectBeta-Fructofuranosidaseen_US
dc.subjectPrebioticsen_US
dc.subjectFructo-oligosaccharidesen_US
dc.subjectFosen_US
dc.subjectNutraceuticalsen_US
dc.subjectFunctional Foodsen_US
dc.titlePurification and kinetic characterization of a fructosyltransferase from Aspergillus aculeatusen_US
dc.typeartículoen_US
dc.identifier.doihttp://dx.doi.org/10.1016/j.jbiotec.2006.09.017-
dc.description.peerreviewedPeer revieweden_US
dc.relation.publisherversionhttp://dx.doi.org/10.1016/j.jbiotec.2006.09.017en_US
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