English
español
Please use this identifier to cite or link to this item:
http://hdl.handle.net/10261/8021
Share/Impact:
Statistics |
![]() ![]() ![]() |
|
|
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |||
|
Title: | Purification and kinetic characterization of a fructosyltransferase from Aspergillus aculeatus |
Authors: | Ghazi, Iraj ![]() ![]() ![]() ![]() ![]() |
Keywords: | Beta-Fructofuranosidase Prebiotics Fructo-oligosaccharides Fos Nutraceuticals Functional Foods |
Issue Date: | 2007 |
Publisher: | Elsevier |
Citation: | Journal of Biotechnology Volume 128, Issue 1, 30 January 2007, Pages 204–211 |
Abstract: | A fructosyltransferase present in Pectinex Ultra SP-L, a commercial enzyme preparation from Aspergillus aculeatus, was purified to 107-fold and further characterised. The enzyme was a dimeric glycoprotein (20% w/w carbohydrate content) with a molecular mass of around 135 kDa for the dimer. Optimal activity/stability was found in the pH range 5.0-7.0 and at 60ºC. It was stable or slightly activated (up to 1.4-fold) in the presence of reducing agents such as dithiotreitol and 2-mercaptoethanol, and detergents such as sodium dodecylsulphate and Tween 80. The enzyme was able to transfer fructosyl groups from sucrose as donor producing the corresponding series of fructooligosaccharides: 1-kestose, nystose and fructosylnystose. Using sucrose as substrate, the kcat and Km values for transfructosylating activity were 1.62 0.09 104 s-1 and 0.53 0.05 M, whereas for hydrolytic activity the corresponding values were 775 25 s-1 and 27 3 mM. At elevated sucrose concentrations, the fructosyltransferase from A. aculeatus showed a high transferase/hydrolase ratio that confers it a great potential for the industrial production of prebiotic fructooligosaccharides. |
Publisher version (URL): | http://dx.doi.org/10.1016/j.jbiotec.2006.09.017 |
URI: | http://hdl.handle.net/10261/8021 |
DOI: | 10.1016/j.jbiotec.2006.09.017 |
Appears in Collections: | (ICP) Artículos |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
postprintJBIOTEC.pdf | 321,79 kB | Adobe PDF | ![]() View/Open |
Show full item record
Review this work
Review this work
Related articles:
WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.