English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/80133
Share/Impact:
Statistics
logo share SHARE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

New mutations of Saccharomyces cerevisiae that partially relieve both glucose and galactose repression activate the protein kinase Snf1

AuthorsRodríguez, Cristina ; Sanz, Pascual ; Gancedo, Carlos
Issue Date2003
PublisherWiley-Blackwell
CitationFEMS Yeast Research 3(1): 77-84 (2003)
AbstractWe isolated from Saccharomyces cerevisiae two mutants, esc1-1 and ESC3-1, in which genes FBP1, ICL1 or GDH2 were partially derepressed during growth in glucose or galactose. The isolation was done starting with a triple mutant pyc1 pyc2 mth1 unable to grow in glucose-ammonium medium and selecting for mutants able to grow in the non-permissive medium. HXT1 and HXT2 which encode glucose transporters were expressed at high glucose concentrations in both esc1-1 and ESC3-1 mutants, while derepression of invertase at low glucose concentrations was impaired. REG1, cloned as a suppressor of ESC3-1, was not allelic to ESC3-1. Two-hybrid analysis showed an increased interaction of the protein kinase Snf1 with Snf4 in the ESC3-1 mutant; this was not due to mutations in SNF1 or SNF4. ESC3-1 did not bypass the requirement of Snf1 for derepression. We hypothesize that ESC3-1 either facilitates activation of Snf1 or interferes with its glucose-dependent inactivation. © 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
URIhttp://hdl.handle.net/10261/80133
DOI10.1111/j.1567-1364.2003.tb00141.x
Identifiersdoi: 10.1111/j.1567-1364.2003.tb00141.x
issn: 1567-1356
e-issn: 1567-1364
Appears in Collections:(IBV) Artículos
(IIBM) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.