English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/80081
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Serum deprivation increases ceramide levels and induces apoptosis in undifferentiated HN9.10e cells

AuthorsColombaioni, Laura; Frago, Laura M.; Varela-Nieto, Isabel ; Pesi, Rossana; García-Gil, Mercedes
Issue Date2002
CitationNeurochemistry International 40(4): 327-336 (2002)
AbstractSphingolipid metabolites have been involved in the regulation of proliferation, differentiation and apoptosis. While cellular mechanisms of these processes have been extensively analysed in the post-mitotic neurons, little is known about proliferating neuronal precursors. We have taken as a model of neuroblasts the embryonic hippocampal cell line HN9.10e. Apoptosis was induced by serum deprivation and by treatment with N-acetylsphingosine (C2-Cer), a membrane-permeant analogue of the second messenger ceramide. Following C2-Cer addition, cytochrome c was released from mitochondria, [Ca2+]i and caspase-3-like activity increased. Both cytochrome c release and rise of [Ca2+]i occurred before caspase-3 activation and nuclear condensation. The intracellular levels of ceramide peaked at 1h following the serum deprivation. These results indicate that the serum deprivation induces a rise in the intracellular ceramide level, and that increased ceramide concentration leads to calcium dysregulation and release of cytochrome c followed by caspase-3 activation. We show that cytochrome c is released without a loss of mitochondrial transmembrane potential. © 2002 Elsevier Science Ltd. All rights reserved.
Identifiersdoi: 10.1016/S0197-0186(01)00090-0
issn: 0197-0186
e-issn: 1872-9754
Appears in Collections:(IIBM) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.