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In vitro methane production by ruminal microorganisms is affected by the diet of donor animals

AuthorsTejido, María L. ; Ranilla, María José ; Saro, C.; Carro Travieso, Mª Dolores
Forage:concentrate ratio
Issue Date2010
PublisherAmerican Dairy Science Association
Poultry Science Association
Asociación Mexicana de Producción Animal
Canadian Society of Animal Science
American Society of Animal Science
CitationAnnual Joint Meeting of the American Dairy Science Association (ADSA-PSA-AMPA) : T376 (2010)
AbstractSix rumen-fistulated sheep were fed 4 diets in a partially replicated 4 × 4 Latin square design to investigate the effects of forage to concentrate ratio (F:C) and type of forage (FOR) in the diet on in vitro methane production. The diets consisted on either 70:30 (HF) or 30:70 (HC) F:C ratio, and either alfalfa hay (A) or grass hay (G) as FOR. In each period, ruminal fluid from each sheep was used to inoculate batch cultures containing the same 4 diets as substrate. Cultures were incubated at 39°C for 24 h. There were no F:C x FOR interactions (P > 0.05) for any measured variable. Methane and total volatile fatty acids (VFA) production was 17.5 and 10.0% times greater (P < 0.05) with HC-inoculum compared with HF-inoculum. Changing the F:C in the diet of sheep did not affect (P > 0.05) propionate production for any substrate, but production of butyrate was augmented (P < 0.01) as F:C increased. Methane:VFA ratio and apparent dry matter digestibility were not affected (P > 0.05) by F:C in the diet of sheep. For all substrates, inoculum from sheep fed A diets promoted greater (P < 0.05) production of methane and total and individual VFA, as well as greater (P < 0.01) acetate:propionate ratios and apparent dry matter digestibility compared with inoculum from sheep fed G diets. Methane:VFA ratio was greater (P < 0.05) with A-inoculum compared with G-inoculum for HC substrates, but no effect of FOR was observed for HF substrates. There were clear differences in methane production among inocula from different sheep, which persisted across diets and substrates. Methane emission estimated from VFA production was about 21% greater (P < 0.001) than that directly measured, but both values were significantly related (r = 0.612; P < 0.001; n = 128). The results indicate that methane production in vitro is affected by both F:C ratio and type of forage in the diet of donors, and these variables should be taken into account when conducting in vitro experiments.
Description1 Página.--Trabajo presentado al 2010 Annual Meeting Abstracts (Denver, Colorado, 11 al 15 de Julio del 2010).--J. Anim. Sci. Vol. 88, E-Suppl. 2/J. Dairy Sci. Vol. 93, E-Suppl. 1/Poult. Sci. Vol. 89, E-Suppl. 1
Publisher version (URL)http://www.jtmtg.org/2010/toc.asp
Appears in Collections:(IGM) Comunicaciones congresos
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