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Purification and characterization of insulin-mimetic inositol phosphoglycan-like molecules from grass pea (Lathyrus sativus) seeds

AuthorsPañeda, Covadonga; Villar, Ana-Victoria; Alonso, Alicia; Goñi, Félix M.; León, Yolanda; Varela-Nieto, Isabel ; Jones, David R.
Issue Date2001
PublisherFeinstein Institute for Medical Research
CitationMolecular Medicine 7(7): 454-460 (2001)
Abstract[Background]: Signal transduction through the hydrolysis of glycosyl-phosphatidylinositol (GPI) leading to the release of the water-soluble inositol phosphoglycan (IPG) molecules has been demonstrated to be important for mediating some of the actions of insulin and insulin-like growth factor-I (IGF-I). [Materials and Methods]: In the present study, GPI from grass pea (Lathyrus sativus) seeds has been purified and partially characterized on the basis of its Chromatographic properties and its compositional analysis. [Results]: The results indicate that it shows similarities to GPI previously isolated from other sources such as rat liver. IPG was generated from L. sativus seed GPI by hydrolysis with a GPI-specific phospholipase D (GPI-PLD). This IPG inhibited protein kinase A (PKA) in an in vitro assay, caused cell proliferation in explanted cochleovestibular ganglia (CVG), and decreased 8-Br-cAMP-induced phosphoenolpyruvate carboxykinase (PEPCK) mRNA expression in cultured hepatoma cells. [Conclusions]: Our data indicate that L. sativus seed IPG possess insulin-mimetic activities. This may explain why L. sativus seeds have been used in some traditional medicines to ameliorate diabetic symptoms.
Description7 páginas, 3 figuras, 2 tablas.-- et al.
Publisher version (URL)http://molmed.org/journal/articles/17/547
Identifiersissn: 1076-1551
e-issn: 1528-3658
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