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Title

Apicomplexa primers amplify Proteromonas (Stramenopiles, Slopalinida, Proteromonadidae) in tissue and blood samples from lizards

AuthorsMaia, Joao P. M. C.; Gómez-Díaz, Elena CSIC ORCID; Harris, David James
KeywordsLizard
Hemogregarine
18S rRNA
Molecular screening
Microscopy
Hemoparasites
Issue DateDec-2012
PublisherPolish Academy of Sciences
CitationActa Parasitologica 57(4): 337-341 (2012)
AbstractMicroscopy has traditionally been the most common method in parasitological studies, but in recent years molecular screening has become increasingly frequent to detect protozoan parasites in a wide range of vertebrate hosts and vectors. During routine molecular screening of apicomplexan parasites in reptiles using the 18S rRNA gene, we have amplified and sequenced Proteromonas parasites from three lizard hosts (less than 1% prevalence). We conducted phylogenetic analysis to confirm the taxonomic position and infer their relationships with other stramenopiles. Although our phylogeny is limited due to scarcity of molecular data on these protists, our results confirm they are closely related to Proteromonas lacertae. Our findings show that unexpected parasites can be amplified from host samples (blood and tissue) using general procedures to detect hemoparasites, and stress that positive PCR amplifications alone should not be considered as definitive proof of infection by particular parasites. Further validation by sequence confirmation and thorough phylogenetic assessment will not only avoid false positives and biased prevalence estimates but also provide valuable information on the biodiversity and phylogenetic relationships of other parasitic organisms. More generally, our results illustrate the perils of general diagnosis protocols in parasitological studies and the need of cross-validation procedures. © 2012 Versita Warsaw and Springer-Verlag Wien.
DescriptionThis work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Publisher version (URL)http://dx.doi.org/10.2478/s11686-012-0048-z
URIhttp://hdl.handle.net/10261/79897
DOI10.2478/s11686-012-0048-z
Identifiersdoi: 10.2478/s11686-012-0048-z
issn: 1230-2821
e-issn: 1896-1851
Appears in Collections:(IBE) Artículos




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