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Vitamin D3 inhibits proliferation and increases c-myc expression in fibroblasts from psoriatic patients

AuthorsCasado, Marta CSIC ORCID ; Martin, M.; Muñoz Terol, Alberto CSIC ORCID; Bernal, Juan CSIC ORCID
Issue Date1998
PublisherEditrice Kurtis
CitationJournal of Endocrinological Investigation 21(8): 520-525 (1998)
AbstractFibroblast cultures were established from the skin of normal and psoriatic subjects. The response to 1α,25-dihydroxyvitamin D3 [1,25-(OH)2D3] of each kind of cells was assessed by measuring tritiated thymidine incorporation into DNA as an index of cell proliferation. We found that both types of cells responded with a similar dose- and time-dependent inhibition of thymidine incorporation. We also studied the response of the mRNA encoding the proto-oncogene c-myc, since its level is associated to the proliferative state in many cell types. Psoriatic fibroblasts contained higher basal amounts of c-myc RNA than control fibroblasts. Addition of 1,25-(OH)2D3 to the culture medium induced a time-dependent increase of c-myc RNA in psoriatic fibroblasts but not in controls. As a control, retinoic acid had no effect in any of the two cell types. It is concluded that in primary normal human fibroblasts, c-myc RNA levels are not correlated with the proliferative state, and that there is an altered expression of this proto-oncogene in psoriasis.
Identifiersissn: 0391-4097
e-issn: 1720-8386
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