English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/7931
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Resistance of rumen bacteria murein to bovine gastric lysozyme

AutorDomínguez-Bello, María G.; Pacheco, María ; Ruiz, Marie C.; Michelangeli, Fabián; Leippe, Matthias; Pedro, Miguel Ángel de
Palabras claveBovine gastric lysozyme
Fecha de publicación11-may-2004
EditorBioMed Central
CitaciónBMC Ecology 2004, 4:7
Resumen[Background] Lysozymes, enzymes mostly associated with defence against bacterial infections, are mureinolytic. Ruminants have evolved a gastric c type lysozyme as a digestive enzyme, and profit from digestion of foregut bacteria, after most dietary components, including protein, have been fermented in the rumen. In this work we characterized the biological activities of bovine gastric secretions against membranes, purified murein and bacteria.
[Results] Bovine gastric extract (BGE) was active against both G+ and G- bacteria, but the effect against Gram- bacteria was not due to the lysozyme, since purified BGL had only activity against Gram+ bacteria. We were unable to find small pore forming peptides in the BGE, and found that the inhibition of Gram negative bacteria by BGE was due to an artefact caused by acetate. We report for first time the activity of bovine gastric lysozyme (BG lysozyme) against pure bacterial cultures, and the specific resistance of some rumen Gram positive strains to BGL.
[Conclusions] Some Gram+ rumen bacteria showed resistance to abomasum lysozyme. We discuss the implications of this finding in the light of possible practical applications of such a stable antimicrobial peptide.
Versión del editorhttp://www.biomedcentral.com/1472-6785/4/7
URIhttp://hdl.handle.net/10261/7931
ISSN1472-6785 (online)
Aparece en las colecciones: (CBM) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
MAdePedro_BMCEcology_7.pdf403,92 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.