Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/7928
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Fas Splicing Regulation during Early Apoptosis Is Linked to Caspase-mediated Cleavage of U2AF65 |
Autor: | Izquierdo, José M. CSIC ORCID | Palabras clave: | U2AF35 U2AF65 |
Fecha de publicación: | 28-may-2008 | Editor: | American Society for Cell Biology | Citación: | Molecular Biology of the Cell, Vol. 19, Issue 8, 3299-3307, August 2008 | Resumen: | U2 small nuclear ribonucleoprotein (snRNP) auxiliary factor 65 kDa (U2AF65) is an essential splicing factor in the recognition of the pre-mRNA 3' splice sites during the assembly of the splicing commitment complex. We report here that U2AF65 is proteolyzed during apoptosis. This cleavage is group I or III caspase dependent in a noncanonical single site localized around the aspartic acid128 residue and leads to the separation of the N- and C-terminal parts of U2AF65. The U2AF65 N-terminal fragment mainly accumulates in the nucleus within nuclear bodies (nucleoli-like pattern) and to a much lesser extent in the cytoplasm, whereas the C-terminal fragment is found in the cytoplasm, even in localization studies on apoptosis induction. From a functional viewpoint, the N-terminal fragment promotes Fas exon 6 skipping from a reporter minigene, by acting as a dominant-negative version of U2AF65, whereas the C-terminal fragment has no significant effect. The dominant-negative behavior of the U2AF65 N-terminal fragment can be reverted by U2AF35 overexpression. Interestingly, U2AF65 proteolysis in Jurkat cells on induction of early apoptosis correlates with the down-regulation of endogenous Fas exon 6 inclusion. Thus, these results support a functional link among apoptosis induction, U2AF65 cleavage, and the regulation of Fas alternative splicing | Descripción: | Supplementary material available | Versión del editor: | http://dx.doi.org/10.1091/mbc.E07-11-1125 | URI: | http://hdl.handle.net/10261/7928 | DOI: | 10.1091/mbc.E07-11-1125 | ISSN: | 1059-1524 | E-ISSN: | 1939-4586 |
Aparece en las colecciones: | (CBM) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
JMIzquierdo_MolBiolCell_3299.pdf | Main text | 1,24 MB | Adobe PDF | Visualizar/Abrir |
JMIzquierdo_MolBiolCell_3299_Supplementary.pdf | 3,41 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
10
checked on 10-abr-2024
SCOPUSTM
Citations
14
checked on 23-abr-2024
WEB OF SCIENCETM
Citations
14
checked on 27-feb-2024
Page view(s)
414
checked on 24-abr-2024
Download(s)
388
checked on 24-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.