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Expression of a yeast gene can be blocked by insertion of short yeast DNA fragments between a UAS and the TATA box

AuthorsVincent, Olivier ; Gancedo, Juana M.
Issue Date1995
CitationCurrent Genetics 27(4): 387-389 (1995)
AbstractWe have constructed a plasmid, pOV10, which facilitates the introduction of putative upstream activating sequences (UAS) or upstream repressing sequences (URS) from yeast genes into plasmids containing CYC1-lacZ fusions, We have observed that the insertion of yeast se quences from 155 to 195 bp between the UAS and the TATA box of a CYC1-lacZ fusion gene can block P-galactosidase expression. It is suggested that this block is related to the formation of nucleosomes on the DNA.
Identifiersdoi: 10.1007/BF00352109
issn: 0172-8083
e-issn: 1432-0983
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