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Title

Development of a Real-Time PCR method for the identification of Atlantic mackerel (Scomber scombrus)

AuthorsVelasco, Amaya ; Sánchez, Ana Cristina ; Martínez, Iciar ; Santaclara, Francisco J. ; Pérez Martín, Ricardo Isaac ; González Sotelo, Carmen
KeywordsAtlantic mackerel
Scomber
Species identification
Pelagic fish
Real-Time PCR
Cytochrome b
Issue Date2013
PublisherElsevier
CitationFood Chemistry 141(3): 2006-2010 (2013)
AbstractA Real Time-PCR method based on TaqMan technology for the identification of Scomber scombrus has been developed. A system of specific primers and a Minor Groove Binding (MGB) TaqMan probe based on sequences of the mitochondrial cytochrome b region was designed. The method was successfully tested in 81 specimens of S. scombrus and related species and validated in 26 different commercial samples. An average Threshold Cycle (Ct) value of 15.3 was obtained with S. scombrus DNA. With the other species tested fluorescence signal was not detected or Ct was significantly higher (P < 0.001). The efficiency of the assay was estimated to be 92.41%, with 100% specificity, and no cross reactivity was detected with any other species. These results reveal that the developed method is a rapid and efficient tool to unequivocally identify S. scombrus and may aid in the prevention of fraud or mislabelling in mackerel products
Description5 páginas, 3 tablas, 2 figuras
Publisher version (URL)http://dx.doi.org/10.1016/j.foodchem.2013.05.077
URIhttp://hdl.handle.net/10261/78850
DOI10.1016/j.foodchem.2013.05.077
ISSN0308-8146
Appears in Collections:(IIM) Artículos
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