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Nicotinamide analogs and DNA-damaging agents deplete thyroid hormone receptor and c-erbA mRNA levels in pituitary GH1 cells

AuthorsSánchez-Pacheco, Aurora; Perez, Paloma; Villa, Aida; Pascual, Ángel CSIC; Aranda, Ana CSIC ORCID
Issue Date1993
CitationMolecular and Cellular Endocrinology 91(1-2): 127-134 (1993)
AbstractIncubation of pituitary GH1 cells with N'-methylnicotinamide, nicotinamide and 3-acetylpyridine which inhibit nuclear ADP-ribosylation and/or the cellular concentration of its substrate NAD+ reduced the amount of nuclear thyroid hormone receptors in a time- and dose-dependent manner without altering the affinity of the receptors for the hormone. A transient activation of poly(ADP-ribose)polymerase by methyl methanesulfonate, ultraviolet irradiation or spermine caused a rapid depletion of cellular NAD+ content and was followed by a strong inhibition of ADP-ribosylation. These agents also produced a very rapid and marked reduction of receptor numbers. The decrease of receptors caused by the different compounds is not secondary to a generalized inhibition of protein synthesis or to an alteration in hormone availability. The abundance of c-erbA α and β mRNAs, which encode thyroid hormone receptors, was reduced in cells treated with the compounds that decrease receptor number, thus suggesting that this effect is caused by a decrease in the expression of c-erbA genes.
Identifiersdoi: 10.1016/0303-7207(93)90264-K
issn: 0303-7207
e-issn: 1872-8057
Appears in Collections:(IIBM) Artículos

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