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Título : Effect of carotenoid deficiency on cells and chlorosomes of Chlorobium phaeobacteroides
Autor : Arellano, Juan B. ; Borrego, Carles M.; Martínez-Planells, Asunción; García-Gil, L. Jesús
Palabras clave : Baseplate
bacteriochlorophyll
carotenoid
chlorosome
CsmA
green sulfur bacteria
Isorenieratene
Fecha de publicación : mar-2001
Editor: Springer
Citación : Archives of Microbiology (2001) 175:226-233
Resumen: The effects of inhibition of carotenoid biosynthesis by 2-hydroxybiphenyl on the photosynthetic growth, pigment composition and chlorosome structure of Chlorobium phaeobacteroides strain CL1401 were examined. At a concentration of 20 µg 2-hydroxybiphenyl ·ml-1, carotenoid synthesis was largely inhibited (85%), but the photosynthetic growth rate was almost unaffected (µcontrol=0.00525-0.00007 h-1 and µHBP-treated=0.00505-0.0005 h-1). Cells grown in the presence of the inhibitor were 5 µm-70 µm long, while control cells were between 2-5 µm long. Moreover, 2-hydroxybiphenyl-treated cells contained fewer, unevenly distributed chlorosomes per µm of cytoplasmic membrane with an irregular arrangement (2.5-1.5 vs of 9.1-1.9). This was concomitant to the 83% decrease in the content of bacteriochlorophyll (BChl) e in 2-hydroxybiphenyl-treated cells. Electron microscopy revealed that the shape of carotenoid-depleted chlorosomes changed from ellipsoidal to spherical, although the mean volume was similar to that of control chlorosomes. SDS-PAGE analysis of the chlorosome polypeptide composition showed that the amount of CsmA protein decreased by 60% in carotenoid-depleted chlorosomes. This was paralleled by a decrease in the baseplate BChl a content. The data suggest that carotenoids are close to the chlorosomal baseplate, where they carry out both structural and photoprotective functions.
Versión del editor: http://www.springerlink.com/content/991fet78j4tu1l2j/fulltext.pdf
URI : http://hdl.handle.net/10261/7846
DOI: 10.1007/s002030100258
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