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The molar extinction coefficient of bacteriochlorophyll e and the pigment stoichiometry in Chlorobium phaeobacteroides

AutorBorrego, Carles M.; Arellano, Juan B. ; Abellà, Carles A.; Gillbro, Tomas; García-Gil, L. Jesús
Palabras claveAntenna pigments
bacteriochlorophyll e
Chlorobium phaeobacteroides
chlorophyll b
chlorosomes
extinction coefficient
green sulfur bacteria
Fecha de publicaciónmay-1999
EditorSpringer
CitaciónPhotosynthesis Research (1999) 60:257-264
ResumenWe have determined the molar extinction coefficient of bacteriochlorophyll (BChl) e, the main light-harvesting pigment from brown-coloured photosynthetic sulfur bacteria. The extinction coefficient was determined using pure[Pr,E]BChl eF isolated by reversed-phase HPLC from crude pigment extracts of Chlorobium (Chl.) phaeobacteroides strain CL1401. The extinction coefficients at the Soret and Qy bands were determined in four organic solvents. The extinction coefficient of BChl e differs from those of other related Chlorobium chlorophylls (BChl c and BChl d) but is similar to that of chlorophyll b. The determined extinction coefficient was used to calculate the stoichiometric BChl e to BChl a and BChl e to carotenoids ratios in whole cells and isolated chlorosomes from Chl. phaeobacteroides strain CL1401 using the spectrum-reconstruction method (SRCM) described by Naqvi et al. (1997) (Spectrochim Acta A Mol Biomol Spectrosc 53: 2229–2234). In isolated chlorosomes, BChl a content was ca. 1% of the total BChl content and the stoichiometric ratio of BChl e to carotenoids was 6. In whole cells,however, BChl a content was 3–4%, owing to the presence of BChl a-containing elements, i.e. FMO protein and reaction centre. An average of 5 BChl e molecules per carotenoid was determined in whole cells.
Versión del editorhttp://www.springerlink.com/content/r626478328j50v6m/
URIhttp://hdl.handle.net/10261/7845
DOI10.1023/A:1006230820007
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