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A novel real-time polymerase chain reaction-based method for the detection and quantification of lactose-fermenting Enterobacteriaceae in the dairy and other food industries

AuthorsMartín, M. Cruz ; Martínez Álvarez, Noelia ; Río Lagar, Beatriz del ; Ladero Losada, Víctor Manuel ; Álvarez González, Miguel Ángel
Issue DateMar-2010
PublisherAmerican Dairy Science Association
CitationJournal of Dairy Science 93(3): 860-867 (2010)
AbstractThe presence of lactose-fermenting Enterobacteriaceae and coliforms is routinely assessed to determine the hygienic quality of water and foods, particularly dairy products. This paper reports the use of lacZ-specific primers in an SYBR green I-based real-time PCR method for the easy and rapid detection of coliforms in dairy products. A large number of bacterial species were assayed to establish the specificity of the method. The sensitivity of the method was assessed using artificially contaminated cheeses. The limit of detection was 1 coliform cell in cheese samples enriched for 8. h in a culture medium. The entire procedure, including sample processing, enrichment, DNA extraction, and real-time PCR amplification, can be completed within 10 to 12. h, making it a single-day assay. © 2010 American Dairy Science Association.
Identifiersdoi: 10.3168/jds.2009-2481
issn: 0022-0302
e-issn: 1525-3198
Appears in Collections:(IPLA) Artículos
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