English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/7829
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 11 veces en Web of Knowledge®  |  Pub MebCentral Ver citas en PubMed Central  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar EndNote (RIS)Exportar EndNote (RIS)
Título : A precise DNA bend angle is essential for the function of the phage 29 transcriptional regulator
Autor : Pérez-Lago, Laura; Salas, Margarita; Camacho, Ana Gema
Palabras clave : Bacteriophage phi 29
Fecha de publicación : 7-ene-2005
Editor: Oxford University Press
Citación : Nucleic Acids Research 2005 33(1):126-134
Resumen: Bacteriophage phi 29 protein p4 is essential for the regulation of the switch from early to late phage transcription. The protein binds to two regions of the phage genome located between the regulated promoters. Each region contains two inverted repeats separated by 1 bp. We used circular permutation assays to study the topology of the DNA upon binding of the protein and found that p4 induced the same extent of bending independent of the topology of the binding region. In addition, the results revealed that the p4-induced bending is not dependent on the affinity to the binding site but is intrinsic to p4 binding. Independent binding sites were identified through the characterization of the minimal sequence required for p4 binding. The protein has different affinity for each of its binding sites, with those overlapping the A2c and A2b promoter cores (sites 1 and 3), having the highest affinity. The functionality of the p4 binding sites and the contribution of p4-mediated promoter restructuring in transcription regulation is discussed
Versión del editor: http://dx.doi.org/10.1093/nar/gki146
URI : http://hdl.handle.net/10261/7829
DOI: 10.1093/nar/gki146
ISSN: 0305-1048 (print)
1362-4962 (online)
Aparece en las colecciones: (CBM) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
MSalas_NAR_126.pdf836,12 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.