English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/78019
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


The N-terminal region of nurr1 (a.a 1-31) is essential for its efficient degradation by the ubiquitin proteasome pathway

AuthorsÁlvarez-Castelao, Beatriz; Losada, Fernando; Ahicart, Patrícia; Castaño, José G.
Issue Date2013
PublisherPublic Library of Science
CitationPLoS ONE 88(2): e55999 (2013)
AbstractNURR1/NR4A2 is an orphan nuclear receptor that is critical for the development and maintenance of mesencephalic dopaminergic neurons and regulates transcription of genes involved in the function of dopaminergic neurons directly via specific NGFI-B response elements (NBRE).and substantial data support a possible role of Nurr1 in the pathogenesis of Parkinson's disease (PD). Here we show that Nurr1 is degraded by the ubiquitin-proteasome pathway and determined that N-terminal region (a.a 1-31) of Nurr1 is essential for an efficient targeting of Nurr1 to degradation in the cell. Nurr1 Δ1-31 has a much longer half-life, and as a consequence its steady-state protein levels were higher, than full-length Nurr1 in the cell. Nurr1 Δ1-31 was as potent as Nurr1 full length in transcriptional luciferase reporter assays after normalization with the corresponding steady-state protein expression levels, either in trans-activation of NBRE or trans-repression of iNOS (inducible NO synthase) reporters. These results suggest that Nurr1 Δ1-31, because of longer persistence in the cell, can be a good candidate for gene and cell therapies in the treatment of PD. © 2013 Alvarez-Castelao et al.
Publisher version (URL)http://dx.doi.org/10.1371/journal.pone.0055999
Identifiersdoi: 10.1371/journal.pone.0055999
issn: 1932-6203
Appears in Collections:(IIBM) Artículos
Files in This Item:
File Description SizeFormat 
The N-Terminal.pdf681,99 kBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.