English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/7788
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


A specific loop in human DNA polymerase mu allows switching between creative and DNA-instructed synthesis

AuthorsJuárez, Raquel; Ruiz, José F. ; Nick McElhinny, Stephanie A.; Ramsden, Dale; Blanco, Luis
KeywordsDNA polymerase mu
Issue Date8-Sep-2006
PublisherOxford University Press
CitationNucleic Acids Research 2006 34(16):4572-4582
AbstractHuman DNA polymerase mu (Polµ) is a family X member that has terminal transferase activity but, in spite of a non-orthodox selection of the template information, displays its maximal catalytic efficiency in DNA-templated reactions. As terminal deoxynucleotidyl transferase (TdT), Polµ has a specific loop (loop1) that could provide this enzyme with its terminal transferase activity. When loop1 was deleted, human Polµ lacked TdT activity but improved DNA-binding and DNA template-dependent polymerization. Interestingly, when loop1 from TdT was inserted in Polµ (substituting its cognate loop1), the resulting chimaera displayed TdT activity, preferentially inserting dGTP residues, but had a strongly reduced template-dependent polymerization activity. Therefore, a specialized loop in Polµ, that could adopt alternative conformations, appears to provide this enzyme with a dual capacity: (i) template independency to create new DNA information, in which loop1 would have an active role by acting as a ‘pseudotemplate’; (ii) template-dependent polymerization, in which loop1 must allow binding of the template strand. Recent in vivo and in vitro data suggest that such a dual capacity could be advantageous to resolve microhomology-mediated end-joining reactions.
Publisher version (URL)http://dx.doi.org/10.1093/nar/gkl457
Appears in Collections:(CBM) Artículos
Files in This Item:
File Description SizeFormat 
LBlanco_NAR_4572.pdf560,46 kBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.