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Transcriptional activation by AP-2 is modulated by the oncogene DEK

AutorCampillos, Mónica; García, Miguel Ángel; Valdivieso Amate, Fernando; Vázquez, Jesús
Palabras claveAP-2 in vitro
APOE promoter
Fecha de publicación2003
EditorOxford University Press
CitaciónNucleic Acids Research, 2003, Vol. 31, No. 5, 1571-1575
ResumenCell differentiation and development are highly regulated processes at the transcriptional level. One of the main transcription factors that regulate these processes is AP-2, a cell-type specific protein required for vertebrate development and embryogenesis. AP-2 also regulates apoptosis and cell-cycle specific events by interacting with the oncogene c-Myc. In searching for novel AP-2- interacting factors, using an affinity chromatography approach, we have observed that oncoprotein DEK interacts with AP-2 in vitro. The existence of an interaction between AP-2 and DEK in cellular cultures was demonstrated by expression of a tagged AP-2 form followed by immunodetection. By transient co-expression experiments using a reporter for APOE promoter activity we have found that DEK stimulates the transactivation activity of AP-2 over APOE promoter. Finally, electrophoretic mobility shift assays suggested that DEK enhances the DNA-binding activity of AP-2. Our data suggest a novel cellular function of DEK as a transcriptional co-activator
Versión del editorhttp://dx.doi.org/10.1093/nar/gkg247
URIhttp://hdl.handle.net/10261/7780
DOI10.1093/nar/gkg247
ISSN0305-1048 (print)
1362-4962 (online)
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