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A generic procedure to monitor Maillard-derived Xuorescent compounds in cookies by Xow-injection analysis

AuthorsCalvarro, J.; Gökmen, V.; Morales, F. J.
Issue Date2009
CitationEuropean Food Research and Technology 229: 843- 851 (2009)
AbstractFlow-injection analysis is proposed for routine measurement of Maillard-derived Xuorescent compounds (FC) in cookies as a marker of the extent of baking. In addition, procedure was applied to investigate the formation of free and total (bound to protein free) FC in cookie-resembling models and in commercial wheat-based cookies as well. FC accounts for the overall Xuorescence response of Maillard-derived Xuorescent compounds (bound or not to protein) formed during baking but not for a single compound. Free and total FC values increased exponentially during baking at 200, 210 and 220 °C and an induction period of 10 min was observed for free FC. In the complex scenario of the progress of the Maillard reaction (MR) during baking, formation of FC (347/415, ext/emm) was not the limiting step for browning development at the advanced stage of the reaction. Furthermore, the formation of Maillard-derived Xuorescent compounds and browning during baking were a consequence of parallel reactions apart from the classical MR scheme of consecutive reactions. Total/free FC ratio was dependent on the baking conditions applied and ratio was signiWcantly decreased at severe baking conditions. Total/free FC ratio could be used as a reference marker for monitoring the process and to identify potential over-processing situations during baking. In addition, Xuorescent residues were originally bound to protein because total/free FC ratio decreased drastically as increased the temperature and time of the process. Levels of total FC were nearly 20-fold of free FC in commercial samples. Values of FC were positively correlated with acrylamide, a Maillard-derived food processing contaminant. © Springer-Verlag 2009.
Identifiersdoi: 10.1007/s00217-009-1119-8
issn: 1438-2377
Appears in Collections:(IF) Artículos
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