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dc.contributor.authorHiguero, Alonso M.-
dc.contributor.authorSánchez-Ruiloba, Lucía-
dc.contributor.authorPortillo, Francisco-
dc.contributor.authorIglesias, Teresa-
dc.date.accessioned2013-05-31T09:54:54Z-
dc.date.available2013-05-31T09:54:54Z-
dc.date.issued2010-
dc.identifierdoi: 10.1074/jbc.M109.024703-
dc.identifierissn: 0021-9258-
dc.identifiere-issn: 1083-351X-
dc.identifier.citationJournal of Biological Chemistry 285(2): 1343-1357 (2010)-
dc.identifier.urihttp://hdl.handle.net/10261/77260-
dc.description.abstractIn order for neurons to perform their function, they must establish a highly polarized morphology characterized, in most of the cases, by a single axon and multiple dendrites. Herein we find that the evolutionarily conserved protein Kidins220 (kinase D-interacting substrate of 220-kDa), also known as ARMS (ankyrin repeat-rich membrane spanning), a downstream effector of protein kinase D and neurotrophin and ephrin receptors, regulates the establishment of neuronal polarity and development of dendrites. Kidins220/ARMS gain and loss of function experiments render severe phenotypic changes in the processes extended by hippocampal neurons in culture. Although Kidins220/ARMS early overexpression hinders neuronal development, its down-regulation by RNA interference results in the appearance of multiple longer axon-like extensions as well as aberrant dendritic arbors. We also find that Kidins220/ARMS interacts with tubulin and microtubule-regulating molecules whose role in neuronal morphogenesis is well established (microtubule-associated proteins 1b, 1a, and 2 and two members of the stathmin family). Importantly, neurons where Kidins220/ARMS has been knocked down register changes in the phosphorylation activity of MAP1b and stathmins. Altogether, our results indicate that Kidins220/ARMS is a key modulator of the activity of microtubule-regulating proteins known to actively regulate neuronal morphogenesis and suggest a mechanism by which it contributes to control neuronal development.-
dc.description.sponsorshipThis work was supported by “Ministerio de Ciencia e Innovación” Grant SAF2008-01951, “Neurodegmodels” Grant CAM S-SAL-0202-2006-01 from “Comunidad de Madrid,” and CIBERNED from “Instituto de Salud Carlos III” (Spain) (to T. I.).-
dc.language.isoeng-
dc.publisherAmerican Society for Biochemistry and Molecular Biology-
dc.rightsclosedAccess-
dc.titleKidins220/ARMS modulates the activity of microtubule-regulating proteins and controls neuronal polarity and development-
dc.typeartículo-
dc.identifier.doi10.1074/jbc.M109.024703-
dc.date.updated2013-05-31T09:54:54Z-
dc.description.versionPeer Reviewed-
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